Tomita Y, Zhang Q W, Uchida T, Yoshikawa M, Shimizu I, Iwai T, Nomoto K, Yasui H
Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Heart Lung Transplant. 2001 Jun;20(6):699-702. doi: 10.1016/s1053-2498(00)00219-9.
A new method of mouse aortic graft transplantation into carotid artery was developed with cuff technique. By harvesting the descending aorta of the donor using a small Teflon cuff (external diameter 0.6 mm, internal diameter 0.4 mm) and super fine-tip forceps, and modifying the method of mouse heterotopic heart transplantation with cuff technique, donor descending aortic allografts could be interposed in the common carotid artery of recipient mice. Histological analysis demonstrated neither evidence of tissue damage nor intimal thickening in isograft implanted over 100 days. We strongly recommend that this new model of aortic transplantation in mice is a simple and useful technique for vascular transplantation research.
采用袖套技术开发了一种将小鼠主动脉移植到颈动脉的新方法。使用小型聚四氟乙烯袖套（外径0.6毫米，内径0.4毫米）和超细头镊子获取供体的降主动脉，并采用袖套技术改进小鼠异位心脏移植方法，可将供体降主动脉同种异体移植物植入受体小鼠的颈总动脉。组织学分析表明，植入超过100天的同基因移植物既没有组织损伤的迹象，也没有内膜增厚。我们强烈推荐这种小鼠主动脉移植的新模型是一种用于血管移植研究的简单且有用的技术。
