Tomita Y, Zhang Q W, Yoshikawa M, Uchida T, Nomoto K, Yasui H
Department of Cardiovascular Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Transplantation. 1997 Dec 15;64(11):1598-601. doi: 10.1097/00007890-199712150-00017.
The method for mouse vascularized heart transplantation have been described using suture and cuff techniques. Technical problems have limited its widespread use. Here, we describe our method of modified cervical heterotopic transplantation with the cuff technique.
By using a smaller Teflon cuff (external diameter 0.6 mm, internal diameter 0.4 mm) and superfine-tip forceps, it became possible to directly pull the edge of the carotid artery and evert the proximal end of the artery over the cuff. Similarly, the external jugular vein could be easily everted over a 22-gauge cuff with this direct pulling method.
By these modifications, the operation time was reduced. It usually takes 20 min for the donor harvest, 15 min for preparation of the cervical vessels, and 15 min for anastomosis. All procedures from the donor harvest through skin closure of the recipient mice can be completed within 1 hr, and ischemic time is within 25-40 min.
This method can be used to investigate cyclophosphamide-induced tolerance and mechanisms of reperfusion injury.
已描述了使用缝合和套管技术进行小鼠血管化心脏移植的方法。技术问题限制了其广泛应用。在此，我们描述了采用套管技术的改良颈部异位移植方法。
通过使用较小的聚四氟乙烯套管（外径0.6毫米，内径0.4毫米）和超细头镊子，能够直接牵拉颈动脉边缘并将动脉近端外翻至套管上。同样，采用这种直接牵拉方法，颈外静脉也能轻松外翻至22号套管上。
通过这些改良，手术时间缩短了。供体获取通常需要20分钟，颈部血管准备需要15分钟，吻合需要15分钟。从供体获取到受体小鼠皮肤缝合的所有操作可在1小时内完成，缺血时间在25至40分钟内。
该方法可用于研究环磷酰胺诱导的耐受性和再灌注损伤机制。
