法国围产期队列中感染HIV-1的新生儿的齐多夫定基因型耐药性

Zidovudine genotypic resistance in HIV-1-infected newborns in the French perinatal cohort.

作者信息

Masquelier B, Chaix M L, Burgard M, Lechenadec J, Doussin A, Simon F, Cottalorda J, Izopet J, Tamalet C, Douard D, Fleury H, Mayaux M J, Blanche S, Rouzioux C

机构信息

Laboratoires de Virologie, Centres Hospitaliers Universitaires de Bordeaux, Hôpital Pellegrin, Place Amelie Raba Léon, 33076 Bordeaux CEDEX France.

出版信息

J Acquir Immune Defic Syndr. 2001 Jun 1;27(2):99-104. doi: 10.1097/00126334-200106010-00001.

DOI:10.1097/00126334-200106010-00001

PMID:11404530

Abstract

A retrospective study was set up to investigate the frequency of zidovudine (ZDV)-resistant HIV-1 in infected newborns after ZDV prophylaxis in the French Perinatal Cohort study. Nucleotide sequence analysis was carried out from 34 infants' isolates and 18 maternal plasma samples. Mutations related to ZDV resistance were found in the HIV-1 reverse transcriptase in 7 of 34 children (20%). Evidence of mother-child transmission of ZDV-resistant HIV-1 was found in 4 cases. Phylogenetic analysis showed that 14 of 34 HIV-1 isolates from the infants belonged to non-B subtypes. The presence of ZDV resistance-encoding mutations in the newborn isolates was associated with a longer total duration of exposure to ZDV. In a context of a wide HIV-1 variability, ZDV resistance can be one of the factors contributing to mother-child transmission.

摘要

在法国围产期队列研究中，开展了一项回顾性研究，以调查接受齐多夫定（ZDV）预防治疗的感染新生儿中对ZDV耐药的HIV-1的出现频率。对34例婴儿的病毒分离株和18份母亲血浆样本进行了核苷酸序列分析。在34名儿童中的7名（20%）的HIV-1逆转录酶中发现了与ZDV耐药相关的突变。在4例病例中发现了母婴传播ZDV耐药HIV-1的证据。系统发育分析表明，34例婴儿的HIV-1分离株中有14株属于非B亚型。新生儿分离株中存在ZDV耐药编码突变与ZDV暴露总时长较长有关。在HIV-1高度变异的情况下，ZDV耐药可能是导致母婴传播的因素之一。