Sadick N S, Hudgins L C
Department of Dermatology, Weill Medical College of Cornell University, New York, NY, USA.
Arch Dermatol. 2001 Jun;137(6):723-7.
To determine whether autologously transferred human adipose tissue maintains viability in vivo for prolonged periods.
Six healthy female patients (mean age, 61.5 years; mean body mass index, 23.4 kg/m2) received autologous fat transplants from the gluteus to the nasolabial folds. Subcutaneous fat was sampled from facial and gluteal sites 4 times in 1 year.
Private practice, basic science research center.
After local anesthesia, 10 g of subcutaneous adipose tissue was harvested from the right buttock of each patient. Ten milligrams of adipose tissue was aspirated from the right nasolabial fold. Five grams of gluteal fat was then injected into each nasolabial fold using a uniform monolayer threading technique with no overcorrection. As controls, 10 mg of adipose tissue was obtained from the opposite left buttock and left cheek. Adipose tissue from the transplanted and control facial and gluteal sites was sampled at 4, 6, and 12 months after transplantation.
Gluteal fat has more monounsaturated fatty acids and less saturated fatty acids than facial fat. This unique site-specific fatty acid pattern was used to assess the course of the survival of transplanted adipose tissue in the nasolabial region. In all fat samples, the percent area (weight percentage) was obtained for each fatty acid (C12:0 to C22:6 omega-3) using capillary gas chromatography. Clinical results were also analyzed by macrophotographs.
As expected, gluteal fat had significantly more monounsaturated fatty acids and less saturated fatty acids than facial fat. In 5 of 6 patients, at 4, 6, and 12 months after transplantation, the fatty acid pattern at the transplanted recipient site was similar to the pattern of the control facial site. However, at 4 months, 1 patient had a fatty acid pattern in the transplant recipient site that was similar to the pattern of her gluteal fat. This pattern persisted for 1 year. Fat retention at the transplant site was corroborated by photographic assessment.
Long-term adipocyte survival is an achievable goal following fat transfer. The importance of harvesting and injection techniques as well as adipose tissue characteristics require further study.
确定自体移植的人体脂肪组织在体内能否长期保持活力。
6名健康女性患者（平均年龄61.5岁；平均体重指数23.4kg/m²）接受了从臀部分至鼻唇沟的自体脂肪移植。在1年内从面部和臀部4次采集皮下脂肪样本。
私人诊所，基础科学研究中心。
局部麻醉后，从每位患者的右臀部采集10g皮下脂肪组织。从右鼻唇沟吸取10mg脂肪组织。然后采用均匀单层穿线技术，不进行过度矫正，将5g臀脂肪注入每个鼻唇沟。作为对照，从对侧左臀部和左脸颊获取10mg脂肪组织。在移植后4、6和12个月对移植和对照的面部及臀部部位的脂肪组织进行采样。
臀脂肪比面部脂肪含有更多的单不饱和脂肪酸和更少的饱和脂肪酸。这种独特的部位特异性脂肪酸模式用于评估鼻唇区域移植脂肪组织的存活过程。在所有脂肪样本中，使用毛细管气相色谱法获取每种脂肪酸（C12:0至C22:6ω-3）的面积百分比（重量百分比）。临床结果也通过宏观照片进行分析。
正如预期的那样，臀脂肪比面部脂肪含有显著更多的单不饱和脂肪酸和更少的饱和脂肪酸。在6名患者中的5名患者中，移植后4、6和12个月，移植受体部位的脂肪酸模式与对照面部部位的模式相似。然而，在4个月时，1名患者移植受体部位的脂肪酸模式与她的臀脂肪模式相似。这种模式持续了1年。通过照片评估证实了移植部位的脂肪保留情况。
脂肪移植后长期的脂肪细胞存活是一个可以实现的目标。采集和注射技术以及脂肪组织特征的重要性需要进一步研究。
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