Garaulet M, Hernandez-Morante J J, Lujan J, Tebar F J, Zamora S
Department of Physiology, University of Murcia, Murcia, Spain.
Int J Obes (Lond). 2006 Jun;30(6):899-905. doi: 10.1038/sj.ijo.0803219.
To evaluate the body fat distribution and fat cell size and number in an overweight/obese population from both genders, and to determine the possible relationship between fat cell data from three different adipose tissue localizations (subcutaneous (SA), perivisceral and omental) and adipose tissue composition and dietary fatty acid.
The sample consisted of 84 overweight/obese patients (29 men and 55 women) who have undergone abdominal surgery. The adipocyte size and total fat cell number was studied. Fat cell data were related with anthropometric, adipose tissue and subject's habitual diet fatty acid composition.
Fat cell size was measured according to a Sjöström method from the three adipose depots. Total fat cell number was also calculated. The fatty acid composition of adipose tissue was examined by gas chromatography. The subjects diet was studied by a 7 days dietary record.
Our data showed a negative relationship between the adipocyte size and the n-6 and n-3 fatty acids content of the SA adipose tissue (r=-0.286, P=0,040; r=-0.300, P=0.030) respectively, and the n-6 in the omental depots (r=-0.407, P=0.049) in the total population. Positive associations with the total of saturated (r=0.357, P=0.045) and negative (r=-0.544, P=0.001) with the n-9 fatty acids were observed when the relationship between the adipocyte number and the fatty acid composition of the different anatomical fat regions was studied. Dietary fatty acids composition positively correlated with fat cell size for the myristic acid (14:0) in men in the visceral depot (r=0.822, P=0.023), and for the saturated fatty acids (SFAs) in women in the omental depot (r=0.486, P=0.035).
In the present study, for the first time in humans we found that n-3 and n-6 fatty acids are related to a reduced adipocyte size according to the depot localization. In contrast, adipose tissue and dietary SFAs significantly correlated with an increase in fat cell size and number. No significant associations were found between n-9 acids content and adipocyte size. However, n-9 adipose tissue fatty acids content was inversely associated with fat cell number showing that this type of fatty acid could limit hyperplasia in obese populations. The differences observed in the three different regions, perivisceral, omental and SA fat, indicate that this population adipose tissue have depot-specific differences.
评估超重/肥胖人群中男女的体脂分布、脂肪细胞大小和数量，并确定来自三种不同脂肪组织定位（皮下、内脏周围和网膜）的脂肪细胞数据与脂肪组织组成和膳食脂肪酸之间的可能关系。
样本包括84名接受腹部手术的超重/肥胖患者（29名男性和55名女性）。研究了脂肪细胞大小和总脂肪细胞数量。脂肪细胞数据与人体测量学、脂肪组织和受试者的习惯性饮食脂肪酸组成相关。
根据Sjöström方法从三个脂肪库测量脂肪细胞大小。还计算了总脂肪细胞数量。通过气相色谱法检测脂肪组织的脂肪酸组成。通过7天饮食记录研究受试者的饮食。
我们的数据显示，在总体人群中，脂肪细胞大小与皮下脂肪组织中n-6和n-3脂肪酸含量分别呈负相关（r=-0.286，P=0.040；r=-0.300，P=0.030），与网膜库中的n-6呈负相关（r=-0.407，P=0.049）。在研究脂肪细胞数量与不同解剖脂肪区域的脂肪酸组成之间的关系时，观察到与饱和脂肪酸总量呈正相关（r=0.357，P=0.045），与n-9脂肪酸呈负相关（r=-0.544，P=0.001）。膳食脂肪酸组成与男性内脏库中肉豆蔻酸（14:0）的脂肪细胞大小呈正相关（r=0.822，P=0.023），与女性网膜库中饱和脂肪酸（SFAs）的脂肪细胞大小呈正相关（r=0.486，P=0.035）。
在本研究中，我们首次在人类中发现，根据脂肪库定位，n-3和n-6脂肪酸与脂肪细胞大小减小有关。相反，脂肪组织和膳食饱和脂肪酸与脂肪细胞大小和数量的增加显著相关。未发现n-9酸含量与脂肪细胞大小之间存在显著关联。然而，n-9脂肪组织脂肪酸含量与脂肪细胞数量呈负相关，表明这种类型的脂肪酸可能会限制肥胖人群中的细胞增生。在内脏周围、网膜和皮下脂肪这三个不同区域观察到的差异表明，该人群的脂肪组织存在特定脂肪库差异。
