嗜碱副球菌新型质粒pALC1复制子区域的表征及序列分析

Characterization and sequence analysis of the replicator region of the novel plasmid pALC1 from Paracoccus alcaliphilus.

作者信息

Bartosik D, Witkowska M, Baj J, Wlodarczyk M

机构信息

Department of Bacterial Genetics, Institute of Microbiology, Warsaw University, Miecznikowa 1, Warsaw, 02-096, Poland.

出版信息

Plasmid. 2001 May;45(3):222-6. doi: 10.1006/plas.2000.1505.

DOI:10.1006/plas.2000.1505

PMID:11407917

Abstract

The replicator region of a low-copy-number plasmid, pALC1, of Paracoccus alcaliphilus JCM 7364 was cloned in a form of the minireplicon pALC100 (3.6 kb). The host range of the minireplicon embraces several species of genus Paracoccus, as well as Agrobacterium tumefaciens, Rhizobium leguminosarum, and Rhodobacter sphaeroides (all belonging to alpha-Proteobacteria), but not Escherichia coli. The complete nucleotide sequence of the replicator region (2276 bp) revealed the presence of one complete open reading frame coding for the 28.4-kDa protein (RepA) with similarity to replication proteins of plasmid pSW500 of Erwinia stewartii and pVS1 of Pseudomonas fluorescens. The iteron-like region was identified upstream of the repA gene and consisted of two clusters of repeated sequences (17 bp long) separated by a putative DnaA box. Analysis of the predicted amino acid sequence of two adjacent incomplete ORFs suggests the localization of repA between genes involved in conjugation (traG) and partitioning (parA) within the pALC1 genome.

摘要

嗜碱副球菌JCM 7364的低拷贝数质粒pALC1的复制区以微型复制子pALC100（3.6 kb）的形式被克隆。该微型复制子的宿主范围包括副球菌属的几个物种，以及根癌土壤杆菌、豌豆根瘤菌和球形红杆菌（均属于α-变形菌纲），但不包括大肠杆菌。复制区的完整核苷酸序列（2276 bp）显示存在一个完整的开放阅读框，编码与斯氏欧文氏菌的质粒pSW500和荧光假单胞菌的pVS1的复制蛋白相似的28.4 kDa蛋白（RepA）。类迭代子区域在repA基因上游被鉴定出来，由两个重复序列簇（17 bp长）组成，中间隔着一个假定的DnaA框。对两个相邻的不完整开放阅读框的预测氨基酸序列分析表明，repA位于pALC1基因组中参与接合（traG）和分配（parA）的基因之间。