使用基于微球的多重免疫测定法同时检测新生儿干血斑样本中针对三种HIV-1抗原的抗体。
Simultaneous measurement of antibodies to three HIV-1 antigens in newborn dried blood-spot specimens using a multiplexed microsphere-based immunoassay.
作者信息
Bellisario R, Colinas R J, Pass K A
机构信息
Division of Genetic Disorders, Wadsworth Center, New York State Department of Health, 12201-0509, Albany, NY, USA.
出版信息
Early Hum Dev. 2001 Aug;64(1):21-5. doi: 10.1016/s0378-3782(01)00167-0.
Abstract
We developed a fluorescent immunoassay to simultaneously measure antibodies to three HIV-1 antigens from newborn dried blood-spot specimens. The multiplexed assay uses fluorescent microspheres and a flow analyzer. The procedure is sensitive, precise and accurate, and can be expanded to simultaneously measure additional multiple analytes from a single specimen.
摘要
我们开发了一种荧光免疫测定法，用于同时检测新生儿干血斑样本中针对三种HIV-1抗原的抗体。这种多重测定法使用荧光微球和流动分析仪。该方法灵敏、精确且准确，并且可以扩展为同时检测单个样本中的多种其他分析物。
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