在肯尼亚临床分离的肺炎克雷伯菌中发现的一种新型质粒编码头孢他啶酶（CTX-M-12）的分子特征
Molecular characterization of a novel plasmid-encoded cefotaximase (CTX-M-12) found in clinical Klebsiella pneumoniae isolates from Kenya.
作者信息
Kariuki S, Corkill J E, Revathi G, Musoke R, Hart C A
机构信息
Centre for Microbiology Research, Kenya Medical Research Institute, Nairobi, Kenya.
出版信息
Antimicrob Agents Chemother. 2001 Jul;45(7):2141-3. doi: 10.1128/AAC.45.7.2141-2143.2001.
Abstract
Nine Klebsiella pneumoniae isolates, six from blood and three from cerebrospinal fluid of newborn babies at Kenyatta National Hospital, Nairobi, Kenya, were analyzed for the mechanism of cephalosporin resistance. By using pulsed-field gel electrophoresis of XbaI-digested chromosomal DNA, all the nine isolates were found to be clonal. PCR and direct sequencing revealed a novel extended-spectrum beta-lactamase, which we designated CTX-M-12. It has a more potent hydrolytic activity against cefotaxime than against ceftazidime and a pI of 9.0 and is encoded on a large self-transferable ca. 160-kbp plasmid.
摘要
对肯尼亚内罗毕肯雅塔国家医院9株肺炎克雷伯菌分离株进行了头孢菌素耐药机制分析，其中6株来自新生儿血液，3株来自脑脊液。通过对经XbaI酶切的染色体DNA进行脉冲场凝胶电泳分析，发现所有9株分离株均为克隆株。PCR和直接测序揭示了一种新型超广谱β-内酰胺酶，我们将其命名为CTX-M-12。它对头孢噻肟的水解活性比对头孢他啶更强，其等电点为9.0，由一个约160kbp的大型自我转移质粒编码。
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