Blum D, Gall D, Cuvelier L, Schiffmann S N
Département de Neurosciences, ULB-Erasme, Bruxelles, Belgium.
Neuroreport. 2001 Jun 13;12(8):1769-72. doi: 10.1097/00001756-200106130-00050.
3-Nitropropionic acid (3NP) is a succinate dehydrogenase inhibitor classically used to create animal models of Huntington's disease (HD). However, the effects of this neurotoxin are highly variable in the Sprague-Dawley rat strain, impeding the interest of such model in neuroprotection assays. In the present study, we found that continuous s.c. infusion of 3NP in the Lewis rat strain produces homogeneous clinical impairments as well as highly reproducible striatal lesions according to their location and size. More especially, using quantitative reconstructions, we have determined, after 5 days of treatment, that the lesion was topologically reproducible in the lateral part of the striatum in all tested rats. Thus, 3NP-treated Lewis rat provides an improved animal model for testing neuroprotective strategies in HD.
3-硝基丙酸（3NP）是一种琥珀酸脱氢酶抑制剂，传统上用于建立亨廷顿舞蹈病（HD）的动物模型。然而，这种神经毒素在斯普拉格-道利大鼠品系中的作用高度可变，这阻碍了该模型在神经保护试验中的应用。在本研究中，我们发现，在刘易斯大鼠品系中持续皮下输注3NP会产生一致的临床损伤，以及根据其位置和大小高度可重复的纹状体损伤。更特别的是，通过定量重建，我们发现在治疗5天后，所有受试大鼠纹状体外侧部分的损伤在拓扑结构上是可重复的。因此，经3NP处理的刘易斯大鼠为测试HD的神经保护策略提供了一种改进的动物模型。
