Błasiak J, Trzeciak A, Dziki A, Ulańska J, Pander B
Department of Molecular Genetics, University of Lodz, Poland.
Gen Physiol Biophys. 2000 Dec;19(4):373-9.
Salts of divalent cadmium are well-known human mutagens and carcinogens. In the present work, the ability of vitamin C to modulate genotoxic effects of cadmium chloride on human lymphocytes was assessed using single cell gel electrophoresis (comet assay). Vitamin C at 20 and 100 micromol/l and cadmium at 5, 30 and 150 micromol/l significantly increased the tail moment of lymphocytes. Vitamin C also increased the tail moment of cells exposed to cadmium. This effect was concentration-dependent: the higher the vitamin C concentration the greater the tail moment. The combined effects of cadmium and vitamin C were more pronounced at all concentrations tested than the sum of the effects of the compounds applied separately (p < 0.05), so cadmium and vitamin C can be considered to have synergistic effects. The results obtained can be partly explained by the participation of cadmium in the Fenton reaction and reduction of its oxidized form by vitamin C.
二价镉盐是众所周知的人类诱变剂和致癌剂。在本研究中，使用单细胞凝胶电泳（彗星试验）评估了维生素C调节氯化镉对人淋巴细胞遗传毒性作用的能力。20和100微摩尔/升的维生素C以及5、30和150微摩尔/升的镉显著增加了淋巴细胞的尾矩。维生素C还增加了暴露于镉的细胞的尾矩。这种效应呈浓度依赖性：维生素C浓度越高，尾矩越大。在所有测试浓度下，镉和维生素C的联合作用比单独应用化合物的作用之和更显著（p < 0.05），因此镉和维生素C可被认为具有协同作用。所得结果部分可以通过镉参与芬顿反应以及维生素C对其氧化形式的还原作用来解释。
