RecA介导的链交换比缺失或插入更容易跨越替换性异源序列。
RecA-mediated strand exchange traverses substitutional heterologies more easily than deletions or insertions.
作者信息
Bucka A, Stasiak A
机构信息
Laboratoire d'Analyse Ultrastructurale, Université de Lausanne, CH-1015 Lausanne-Dorigny, Switzerland.
出版信息
Nucleic Acids Res. 2001 Jun 15;29(12):2464-70. doi: 10.1093/nar/29.12.2464.
Abstract
RecA protein in bacteria and its eukaryotic homolog Rad51 protein are responsible for initiation of strand exchange between homologous DNA molecules. This process is crucial for homologous recombination, the repair of certain types of DNA damage and for the reinitiation of DNA replication on collapsed replication forks. We show here, using two different types of in vitro assays, that in the absence of ATP hydrolysis RecA-mediated strand exchange traverses small substitutional heterologies between the interacting DNAs, whereas small deletions or insertions block the ongoing strand exchange. We discuss evolutionary implications of RecA selectivity against insertions and deletions and propose a molecular mechanism by which RecA can exert this selectivity.
摘要
细菌中的RecA蛋白及其真核同源物Rad51蛋白负责启动同源DNA分子之间的链交换。这一过程对于同源重组、某些类型DNA损伤的修复以及在坍塌的复制叉上重新启动DNA复制至关重要。我们在此使用两种不同类型的体外试验表明，在没有ATP水解的情况下，RecA介导的链交换能够跨越相互作用DNA之间的小的替换性异源序列，而小的缺失或插入则会阻断正在进行的链交换。我们讨论了RecA对插入和缺失的选择性的进化意义，并提出了一种RecA能够发挥这种选择性的分子机制。
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本文引用的文献
1
Radioprobing of a RecA-three-stranded DNA complex with iodine 125: evidence for recognition of homology in the major groove of the target duplex.用碘125对RecA三链DNA复合物进行放射性探测：靶双链体大沟中同源性识别的证据。
J Mol Biol. 2000 Jun 9;299(3):629-40. doi: 10.1006/jmbi.2000.3770.
2
Initiation of genetic recombination and recombination-dependent replication.遗传重组的起始及依赖重组的复制。
Trends Biochem Sci. 2000 Apr;25(4):156-65. doi: 10.1016/s0968-0004(00)01569-3.
3
Mechanism and control of interspecies recombination in Escherichia coli. I. Mismatch repair, methylation, recombination and replication functions.大肠杆菌种间重组的机制与调控。I.错配修复、甲基化、重组与复制功能。
Genetics. 1998 Oct;150(2):533-42. doi: 10.1093/genetics/150.2.533.
4
Role of the human RAD51 protein in homologous recombination and double-stranded-break repair.人类RAD51蛋白在同源重组和双链断裂修复中的作用。
Trends Biochem Sci. 1998 Jul;23(7):247-51. doi: 10.1016/s0968-0004(98)01232-8.
5
RadA protein is an archaeal RecA protein homolog that catalyzes DNA strand exchange.RadA蛋白是一种古细菌RecA蛋白同源物，可催化DNA链交换。
Genes Dev. 1998 May 1;12(9):1248-53. doi: 10.1101/gad.12.9.1248.
6
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Genes Dev. 1998 Apr 15;12(8):1134-44. doi: 10.1101/gad.12.8.1134.
7
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8
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The duplex DNA is very underwound in the three-stranded RecA protein-mediated synaptic complex.在三链RecA蛋白介导的突触复合体中，双链DNA的缠绕程度很低。
Genes Cells. 1997 May;2(5):303-14. doi: 10.1046/j.1365-2443.1997.1240322.x.
10
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