嗜热乳酸菌的胞外多糖产生菌株根据其胞外多糖结构聚类成不同的组。
Exopolysaccharide-producing strains of thermophilic lactic acid bacteria cluster into groups according to their EPS structure.
作者信息
Marshall V M, Laws A P, Gu Y, Levander F, Rådström P, De Vuyst L, Degeest B, Vaningelgem F, Dunn H, Elvin M
机构信息
Centre for Biomolecular Sciences, University of Huddersfield, UK.
出版信息
Lett Appl Microbiol. 2001 Jun;32(6):433-7. doi: 10.1046/j.1472-765x.2001.00937.x.
AIMS
To compare galactose-negative strains of Streptococcus thermophilus and Lactobacillus delbrueckii subspecies bulgaricus isolated from fermented milk products and known to produce exopolysaccharides (EPSs).
METHODS AND RESULTS
The structures of the EPSs were determined using nuclear magnetic resonance (NMR) and their genetic relationships determined using restriction endonuclease analysis (REA) and random amplification of polymorphic DNA (RAPD). Similar groupings were apparent by REA and RAPD, and each group produced an EPS with a particular subunit structure.
CONCLUSION
Although none of the strains assimilated galactose, all inserted a high proportion of galactose into their EPS when grown in skimmed milk, and fell into three distinct groups.
SIGNIFICANCE AND IMPACT OF THE STUDY
This information should help in an understanding of genetic exchanges in lactic acid bacteria.
目的
比较从发酵乳制品中分离出的、已知能产生胞外多糖（EPS）的嗜热链球菌和德氏保加利亚乳杆菌的半乳糖阴性菌株。
方法与结果
利用核磁共振（NMR）确定EPS的结构，并利用限制性内切酶分析（REA）和随机扩增多态性DNA（RAPD）确定它们的遗传关系。通过REA和RAPD可明显看出相似的分组情况，且每组都产生具有特定亚基结构的EPS。
结论
尽管这些菌株均不能同化半乳糖，但在脱脂牛奶中生长时，它们都将高比例的半乳糖插入其EPS中，并分为三个不同的组。
研究的意义与影响
该信息应有助于理解乳酸菌中的基因交换。
Zotero 插件