菊粉能提高德氏乳杆菌保加利亚亚种 LDB-C1 胞外多糖的合成。
Inulin increases the EPS biosynthesis of Lactobacillus delbrueckii ssp. bulgaricus LDB-C1.
机构信息
Department of Food Nutrition and Health, School of Medicine and Health, Harbin Institute of Technology, Harbin, 150090, People's Republic of China.
Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin, 150010, People's Republic of China.
出版信息
Biotechnol Lett. 2023 Jun;45(5-6):639-654. doi: 10.1007/s10529-023-03365-z. Epub 2023 Apr 3.
OBJECTIVE
Its eps gene cluster, the antioxidant activity and monosaccharide composition of exopolysaccharides, the expression levels of related genes at different fermentations were analyzed for clarifying the exopolysaccharide biosynthesis mechanism of Lactobacillus delbrueckii subsp. bulgaricus LDB-C1.
RESULTS
The comparison analysis of eps gene clusters indicated that the gene clusters present diversity and strain specificity. The crude exopolysaccharides from LDB-C1 exhibited a good antioxidant activity. Compared with glucose, fructose, galactose, and fructooligosaccharide, inulin significantly improved the exopolysaccharide biosynthesis. The structures of EPSs were significantly different under different carbohydrate fermentation conditions. Inulin obviously increased the expressions of most EPS biosynthesis related genes at fermentation 4 h.
CONCLUSION
Inulin accelerated the beginning of the exopolysaccharide production in LDB-C1, and the enzymes promoted by inulin was beneficial for the accumulation of exopolysaccharide at the whole fermentation process.
目的
分析保加利亚乳杆菌 LDB-C1 的 eps 基因簇、胞外多糖的抗氧化活性和单糖组成，以及不同发酵阶段相关基因的表达水平，以阐明其胞外多糖生物合成机制。
结果
eps 基因簇的比较分析表明，基因簇具有多样性和菌株特异性。LDB-C1 的粗胞外多糖具有良好的抗氧化活性。与葡萄糖、果糖、半乳糖和果寡糖相比，菊粉显著提高了胞外多糖的生物合成。在不同碳水化合物发酵条件下，EPS 的结构有显著差异。菊粉明显增加了发酵 4 h 时大多数 EPS 生物合成相关基因的表达。
结论
菊粉加速了 LDB-C1 胞外多糖的生产开始，菊粉促进的酶有利于整个发酵过程中胞外多糖的积累。
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