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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Russell S M, Davey J, Mayer R J

Biochem J. 1979 Jul 1;181(1):15-20. doi: 10.1042/bj1810015.

Antiserum raised to purified human liver monoamine oxidase was used to characterize the monoamine oxidase from human liver, brain cortex, placenta and platelets. 2. Antibodies to monoamine oxidase were purified by adsorption with a mitochondrial preparation. 3. Monoamine oxidase was present in liver particle-free supernatant as measured by enzyme activity and immunodiffusion. 4. Multiple precipitin lines were obtained on immunodiffusion analysis against the purified liver enzyme. It is proposed that this is due to either aggregation or to differential lipid binding. 5. The results suggest that the functionally different enzymes found in liver, brain cortex, platelets and placenta are immunochemically related and may be identical.

用针对纯化的人肝单胺氧化酶产生的抗血清来鉴定人肝、大脑皮层、胎盘和血小板中的单胺氧化酶。2. 单胺氧化酶抗体通过用线粒体制剂吸附来纯化。3. 通过酶活性和免疫扩散测定，单胺氧化酶存在于肝无颗粒上清液中。4. 在针对纯化的肝酶的免疫扩散分析中获得了多条沉淀线。有人提出这是由于聚集或不同的脂质结合所致。5. 结果表明，在肝、大脑皮层、血小板和胎盘中发现的功能不同的酶在免疫化学上相关且可能相同。

Russell S M, Davey J, Mayer R J

Biochem J. 1979 Jul 1;181(1):15-20. doi: 10.1042/bj1810015.

Antiserum raised to purified human liver monoamine oxidase was used to characterize the monoamine oxidase from human liver, brain cortex, placenta and platelets. 2. Antibodies to monoamine oxidase were purified by adsorption with a mitochondrial preparation. 3. Monoamine oxidase was present in liver particle-free supernatant as measured by enzyme activity and immunodiffusion. 4. Multiple precipitin lines were obtained on immunodiffusion analysis against the purified liver enzyme. It is proposed that this is due to either aggregation or to differential lipid binding. 5. The results suggest that the functionally different enzymes found in liver, brain cortex, platelets and placenta are immunochemically related and may be identical.

用针对纯化的人肝单胺氧化酶产生的抗血清来鉴定人肝、大脑皮层、胎盘和血小板中的单胺氧化酶。2. 单胺氧化酶抗体通过用线粒体制剂吸附来纯化。3. 通过酶活性和免疫扩散测定，单胺氧化酶存在于肝无颗粒上清液中。4. 在针对纯化的肝酶的免疫扩散分析中获得了多条沉淀线。有人提出这是由于聚集或不同的脂质结合所致。5. 结果表明，在肝、大脑皮层、血小板和胎盘中发现的功能不同的酶在免疫化学上相关且可能相同。