人单胺氧化酶在线粒体外膜中的向量取向。
The vectorial orientation of human monoamine oxidase in the mitochondrial outer membrane.
作者信息
Russell S M, Davey J, Mayer R J
出版信息
Biochem J. 1979 Jul 1;181(1):7-14. doi: 10.1042/bj1810007.
Abstract
- The localization of monoamine oxidase in the mitochondrial outer membrane was studied in preparations of human liver mitochondrial and brain-cortex non-synaptosomal and synaptosomal mitochondria. 2. Immunochemical accessibility in iso-osmotic and hypo-osmotic mitochondrial preparations was used to localize the enzyme. 3. It was shown that the immunochemically accessible tyramine-oxidizing activity was distributed approximately equally on both surfaces of the membrane in human liver and brain-cortex non-synaptosomal mitochondria. However, the immunochemically accessible beta-phenethylamine-oxidizing activity was situated predominantly on the outer surface, and the immunochemically accessible 5-hydroxytryptamine-oxidizing activity was situated predominantly on the inner surface of the mitochondrial outer membrane in liver and brain-cortex non-synaptosomal mitochondrial preparations. 4. Considerable variation in the distribution of the enzyme in preparations of synaptosomal mitochondria was seen. 5. The simplest model consistent with our observations is that, in liver and brain-cortex non-synaptosomal mitochondria, the tyramine-oxidizing activity is distributed on both sides of the mitochondrial outer membrane, the beta-phenethylamine-oxidizing activity is located on the outer surface of the outer membrane and the 5-hydroxytryptamine-oxidizing activity is located on the inner surface of the mitochondria outer membrane.
摘要
- 利用人肝脏线粒体以及大脑皮质非突触体线粒体和突触体线粒体的制备物，研究了单胺氧化酶在线粒体外膜中的定位。2. 采用等渗和低渗线粒体制备物中的免疫化学可及性来定位该酶。3. 结果表明，在人肝脏和大脑皮质非突触体线粒体中，免疫化学可及的酪胺氧化活性在膜的两侧分布大致相等。然而，在肝脏和大脑皮质非突触体线粒体制备物中，免疫化学可及的β-苯乙胺氧化活性主要位于外表面，而免疫化学可及的5-羟色胺氧化活性主要位于线粒体外膜的内表面。4. 在突触体线粒体的制备物中，观察到该酶的分布存在相当大的差异。5. 与我们的观察结果一致的最简单模型是，在肝脏和大脑皮质非突触体线粒体中，酪胺氧化活性分布在线粒体外膜的两侧，β-苯乙胺氧化活性位于外膜的外表面，5-羟色胺氧化活性位于线粒体外膜的内表面。
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