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火鸡蛋外壳表面异似蛔线虫卵的存在与检出情况

Presence and recovery of Ascaridia dissimilis ova on the external shell surface of turkey eggs.

作者信息

Seaton E M, Monahan C M, Morishita T Y

机构信息

Department of Veterinary Preventive Medicine, The Ohio State University, Columbus 43210, USA.

出版信息

Avian Dis. 2001 Apr-Jun;45(2):500-3.

PMID:11417835
Abstract

Ascaridia dissimilis, a roundworm in turkeys, has been noted with increased frequency in commercial turkeys. Because infected turkeys can shed A. dissimilis ova in their feces, the potential exists for the external surface of turkey eggshells to be contaminated with A. dissimilis ova. The objectives of this study were to determine the presence of and recover A. dissimilis ova on the external surface of the turkey egg. In Experiment 1, turkey eggs were collected from naturally infected flocks, and eggs were processed by a sodium hydroxide procedure to recover any A. dissimilis ova on the external egg surface. In Experiment 2, the external surface of the turkey eggs was inoculated with 116 A. dissimilis ova/g feces, and eggshells were sampled every 3 days until 28 days of incubation to assess the recovery of A. dissimilis ova from the eggshell. In Experiment 1, of the 36 eggs examined from a flock naturally infected with A. dissimilis, one egg had an A. dissimilis ovum on its external eggshell surface. Experiment 2 demonstrated that A. dissimilis ova can be recovered from the external egg surface after a 28-day incubation period in the incubator. Ova recovery declined from an average of 62 A. dissimilis ova/turkey egg at day 2 of incubation to an average of 3 A. dissimilis ova/turkey egg at day 28 of incubation.

摘要

异刺蛔线虫是火鸡体内的一种蛔虫,在商业养殖的火鸡中,其感染频率有所增加。由于受感染的火鸡会在粪便中排出异刺蛔线虫卵,火鸡蛋壳的外表面有可能被异刺蛔线虫卵污染。本研究的目的是确定火鸡蛋外表面是否存在异刺蛔线虫卵并进行回收。在实验1中,从自然感染的鸡群中收集火鸡蛋,通过氢氧化钠处理程序对鸡蛋进行处理,以回收鸡蛋外表面的任何异刺蛔线虫卵。在实验2中,用火鸡每克粪便含116个异刺蛔线虫卵的粪便接种火鸡蛋的外表面,每隔3天对蛋壳进行采样,直至孵化28天,以评估从蛋壳中回收异刺蛔线虫卵的情况。在实验1中,从自然感染异刺蛔线虫的鸡群中检查的36个鸡蛋中,有一个鸡蛋的外蛋壳表面有一个异刺蛔线虫卵。实验2表明,在孵化器中孵化28天后,可以从鸡蛋外表面回收异刺蛔线虫卵。孵化第2天,每枚火鸡蛋回收的虫卵平均数量为62个,到孵化第28天,每枚火鸡蛋回收的虫卵平均数量降至3个。

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