Kuratli S, Hemphill A, Lindh J, Smith D F, Connolly B
Institute of Parasitology, University of Bern, CH-3012, Bern, Switzerland.
Mol Biochem Parasitol. 2001 Jul;115(2):199-208. doi: 10.1016/s0166-6851(01)00287-0.
The Trichinella tsJ5 gene is preferentially expressed in muscle larvae of Trichinella spiralis and encodes a novel protein. Previous observations have shown tsJ5 to be expressed at higher levels in encapsulating species than in non-encapsulating species and down-regulation of gene expression in T. pseudospiralis to be correlated with a lower protein abundance in the muscle larva of this species. In the present study we have determined the full-length cDNA sequence of the tsJ5 homologue in T. pseudospiralis (tpJ5). Antigens recognised by an anti-J5 antibody are found on the cuticular surface of both T. spiralis and T. pseudospiralis muscle larvae, as well as in the body wall muscle. We show that both the TSJ5 and TPJ5 proteins are found in the excretory/secretory fractions collected from muscle larva cultured in vitro and that despite the absence of a typical N-terminal signal sequence, secretion of pTSJ5 is mediated through the classical ER/Golgi secretory pathway.
旋毛虫tsJ5基因在旋毛形线虫的肌幼虫中优先表达，并编码一种新的蛋白质。先前的观察表明，tsJ5在形成包囊的物种中比在不形成包囊的物种中表达水平更高，并且伪旋毛虫中基因表达的下调与该物种肌幼虫中较低的蛋白质丰度相关。在本研究中，我们确定了伪旋毛虫（tpJ5）中tsJ5同源物的全长cDNA序列。抗J5抗体识别的抗原存在于旋毛形线虫和伪旋毛虫肌幼虫的角质层表面以及体壁肌肉中。我们发现，TSJ5和TPJ5蛋白都存在于从体外培养的肌幼虫收集的排泄/分泌部分中，并且尽管缺乏典型的N端信号序列，但pTSJ5的分泌是通过经典的内质网/高尔基体分泌途径介导的。
