Bingham S, Schiff J A
Biochim Biophys Acta. 1979 Sep 11;547(3):531-43. doi: 10.1016/0005-2728(79)90032-x.
Using sulfolipid to locate plastid thylakoid membranes in gradients from dark-grown resting cells it has been possible to study the plastid thylakoid membrane polypeptides of Euglena gracilis var. bacillaris undergoing light-induced chloroplast development. All plastid thylakoid bands seen in dark-growing wild-type cells and in mutant W3BUL in which plastid DNA is undetectable, are observed to increase in amount during plastid development. Others, which are undetectable in dark-grown wild-type and W3BUL increase greatly during plastid development and appear to be those associated with pigment-protein complexes. The data obtained from experiments where the polypeptides were labeled with 35S during development, either continuously or in pulses, were consistent with these findings. Cycloheximide strongly inhibited the increases in amount in all bands and chloramphenicol or streptomycin produced a lower level of inhibition in all bands indicating tight control of theformation of each plastid membrane constituent by the others. The formation of a polypeptide band of 25 000 molecular weight, thought to be a part of a pigment-protein complex of the thylakoid, and chlorophyll synthesis were inhibited identically by these antibiotics.
利用硫脂在黑暗生长的静止细胞梯度中定位质体类囊体膜，从而有可能研究纤细裸藻杆菌变种在光诱导叶绿体发育过程中的质体类囊体膜多肽。在黑暗生长的野生型细胞以及无法检测到质体DNA的突变体W3BUL中看到的所有质体类囊体条带，在质体发育过程中其数量均会增加。其他在黑暗生长的野生型和W3BUL中无法检测到的条带，在质体发育过程中大量增加，似乎是与色素 - 蛋白质复合物相关的条带。从在发育过程中用35S连续或脉冲标记多肽的实验中获得的数据与这些发现一致。放线菌酮强烈抑制所有条带数量的增加，氯霉素或链霉素对所有条带的抑制作用较低，这表明每种质体膜成分的形成受到其他成分的严格控制。这些抗生素对被认为是类囊体色素 - 蛋白质复合物一部分的25000分子量多肽条带的形成和叶绿素合成的抑制作用相同。
