Ward T J, Skow L C, Gallagher D S, Schnabel R D, Nall C A, Kolenda C E, Davis S K, Taylor J F, Derr J N
Department of Veterinary Pathobiology, Texas A & M University, College Station, TX, USA.
Anim Genet. 2001 Apr;32(2):89-91. doi: 10.1046/j.1365-2052.2001.00736.x.
Historical hybridization between Bison bison (bison) and Bos taurus (cattle) has been well documented and resulted in cattle mitochondrial DNA (mtDNA) introgression, previously identified in six different bison populations. In order to examine Y chromosome introgression, a microsatellite marker (BYM-1) with non-overlapping allele size distributions in bison and cattle was isolated from a bacterial artificial chromosome (BAC) clone, and was physically assigned to the Y chromosome by fluorescence in situ hybridization. BYM-1 genotypes for a sample of 143 male bison from 10 populations, including all six populations where cattle mtDNA haplotypes were previously identified, indicated that cattle Y chromosome introgression had not occurred in these bison populations. The differential permeability of uniparentally inherited markers to introgression is consistent with observations of sterility among first generation hybrid males and a sexual asymmetry in the direction of hybridization favouring matings between male bison and female cattle.
美洲野牛（Bison bison）与家牛（Bos taurus）之间的历史杂交已有充分记载，且导致了家牛线粒体DNA（mtDNA）渗入，此前在六个不同的野牛种群中已发现这种情况。为了检测Y染色体渗入情况，从一个细菌人工染色体（BAC）克隆中分离出一个在野牛和家牛中等位基因大小分布不重叠的微卫星标记（BYM-1），并通过荧光原位杂交将其物理定位到Y染色体上。对来自10个种群的143头雄性野牛样本（包括之前鉴定出家牛mtDNA单倍型的所有六个种群）进行的BYM-1基因分型表明，这些野牛种群中未发生家牛Y染色体渗入。单亲遗传标记对渗入的不同渗透性与第一代杂交雄性不育的观察结果以及杂交方向上有利于雄性野牛与雌性家牛交配的性不对称现象一致。
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