Verkaar E L C, Vervaecke H, Roden C, Romero Mendoza L, Barwegen M W, Susilawati T, Nijman I J, Lenstra J A
Faculty of Veterinary Medicine, Utrecht University, Yalelaan 8, 3584 CL Utrecht, The Netherlands.
Heredity (Edinb). 2003 Dec;91(6):565-9. doi: 10.1038/sj.hdy.6800359.
The genetic integrity of crossfertile bovine- or cattle-like species may be endangered by species hybridization. Previously, amplified fragment length polymorphism, satellite fragment length polymorphism and microsatellite assays have been used to analyze the species composition of nuclear DNA in taurine cattle, zebu, banteng and bison populations, while mitochondrial DNA reveals the origin of the maternal lineages. Here, we describe species-specific markers of the paternally transmitted Y-chromosome for the direct detection of male-mediated introgression. Convenient PCR-restriction fragment length polymorphism and competitive PCR assays are shown to differentiate the Y-chromosomes of taurine cattle, American bison and European bison, and to detect the banteng origin of Indonesian Madura and Bali cattle bulls.
可杂交的牛类或类似牛的物种的遗传完整性可能会因物种杂交而受到威胁。此前，扩增片段长度多态性、卫星片段长度多态性和微卫星分析已被用于分析瘤牛、瘤牛、爪哇牛和野牛群体中核DNA的物种组成，而线粒体DNA则揭示了母系谱系的起源。在此，我们描述了父系遗传的Y染色体的物种特异性标记，用于直接检测雄性介导的基因渗入。简便的聚合酶链反应-限制性片段长度多态性和竞争性聚合酶链反应分析被证明可区分瘤牛、美洲野牛和欧洲野牛的Y染色体，并检测印度尼西亚马都拉牛和巴厘岛公牛的爪哇牛起源。
