Moss R B, Giermakowska W K, Wallace M R, Jensen F C, Maigetter R Z, Carlo D J
The Immune Response Corporation, Carlsbad, CA, USA.
Clin Exp Immunol. 2001 May;124(2):248-54. doi: 10.1046/j.1365-2249.2001.01534.x.
We examined HIV-1 antigen specific intracellular expression of perforin on CD4+ and CD8+ lymphocytes in subjects with chronic HIV-1 infection on antiviral drug therapy after immunization with a gp120-depleted, whole killed HIV-1 immunogen (inactivated, gp120-depleted HIV-1 in IFA, REMUNE). Based upon previous results, we hypothesized that the restoration of adequate T helper immune responses by vaccination against HIV-1 could result in the augmentation of CD8+ lymphocyte immune responses measured as perforin expression. In the current study we observed an increase in the frequency of perforin in CD8+ lymphocytes in HIV infected individuals immunized with a gp120-depleted HIV-1 immunogen while on antiviral drug therapy. Furthermore, the frequency of HIV-specific CD8+ perforin expressing cells correlated with the T helper immune response as measured by the lymphocyte proliferative response (LPR). The induction of such responses with immunization may have direct antiviral consequences and is being studied in ongoing clinical trials.
我们检测了慢性HIV-1感染且正在接受抗病毒药物治疗的受试者，在用去除gp120的全灭活HIV-1免疫原（在不完全弗氏佐剂中的灭活、去除gp120的HIV-1，REVIMUNE）免疫后，CD4+和CD8+淋巴细胞上穿孔素的HIV-1抗原特异性细胞内表达。基于先前的结果，我们推测通过针对HIV-1的疫苗接种恢复足够的T辅助免疫反应，可能会导致以穿孔素表达衡量的CD8+淋巴细胞免疫反应增强。在当前研究中，我们观察到在用去除gp120的HIV-1免疫原免疫且正在接受抗病毒药物治疗的HIV感染个体中，CD8+淋巴细胞中穿孔素的频率增加。此外，HIV特异性CD8+穿孔素表达细胞的频率与通过淋巴细胞增殖反应（LPR）测量的T辅助免疫反应相关。免疫诱导的此类反应可能具有直接的抗病毒作用，目前正在正在进行的临床试验中进行研究。
