赫尔墨斯介导的地中海实蝇（Ceratitis capitata）种系转化

Hermes-mediated germ-line transformation of the Mediterranean fruit fly Ceratitis capitata.

作者信息

Michel K, Stamenova A, Pinkerton A C, Franz G, Robinson A S, Gariou-Papalexiou A, Zacharopoulou A, O'Brochta D A, Atkinson P W

机构信息

Department of Entomology, University of California, Riverside, CA 92521, USA.

出版信息

Insect Mol Biol. 2001 Apr;10(2):155-62. doi: 10.1046/j.1365-2583.2001.00250.x.

DOI:10.1046/j.1365-2583.2001.00250.x

PMID:11422511

Abstract

We report the use of the Hermes transposable element for germ-line transformation of the Mediterranean fruit fly, Ceratitis capitata. Hermes was able to genetically transform this insect at an estimated frequency between 0.6 and 1.1%, which is comparable to the transformation frequencies obtained for this species when using other transposable elements. Hermes integrates into the medfly genome by a cut-and-paste mechanism and the sequences integrated into the genome are delimited by the terminal nucleotides of the Hermes inverted terminal repeats. Integration resulted in the generation of 8 bp target site duplications, the sequences of which conformed to the target site duplications generated by hAT element transposition in insects. The Hermes element is one additional genetic tool that can be deployed in manipulating and characterizing the medfly genome.

摘要

我们报道了使用赫耳墨斯转座元件对地中海实蝇（Ceratitis capitata）进行种系转化的情况。赫耳墨斯能够以估计介于0.6%至1.1%之间的频率对这种昆虫进行遗传转化，这与使用其他转座元件时该物种所获得的转化频率相当。赫耳墨斯通过剪切粘贴机制整合到地中海实蝇基因组中，整合到基因组中的序列由赫耳墨斯反向末端重复序列的末端核苷酸界定。整合导致产生了8个碱基对的靶位点重复序列，其序列与昆虫中hAT元件转座产生的靶位点重复序列一致。赫耳墨斯元件是又一种可用于操纵和表征地中海实蝇基因组的遗传工具。