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利用海德氏果蝇转座元件将基因导入地中海实蝇（Ceratitis capitata）。

Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element.

作者信息

Loukeris T G, Livadaras I, Arcà B, Zabalou S, Savakis C

机构信息

Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Crete, Greece.

出版信息

Science. 1995 Dec 22;270(5244):2002-5. doi: 10.1126/science.270.5244.2002.

DOI:10.1126/science.270.5244.2002
PMID:8533094
Abstract

Exogenous functional DNA was introduced into the germline chromosomes of the Mediterranean fruit fly (medfly) Ceratitis capitata with a germline transformation system based on the transposable element Minos from Drosophila hydei. Transformants were identified as phenotypic revertants of a white-eyed mutation carried by the recipient strain. Clusters of transformants were detected among the progeny of 390 individuals screened for germline transformation. Five independent and phenotypically active integration events were identified, in each of which a single copy of the transposon was inserted into a different site of the medfly genome. Molecular analysis indicates that they represent transposase-mediated insertions of the transposon into medfly chromosomes.

摘要

利用基于果蝇海德氏果蝇转座因子Minos的种系转化系统，将外源性功能性DNA导入地中海实蝇（medfly）Ceratitis capitata的种系染色体中。转化体被鉴定为受体菌株携带的白眼突变的表型回复体。在筛选种系转化的390个个体的后代中检测到转化体簇。鉴定出五个独立且具有表型活性的整合事件，在每个事件中，转座子的单拷贝插入到地中海实蝇基因组的不同位点。分子分析表明，它们代表转座酶介导的转座子插入地中海实蝇染色体。

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Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element.利用海德氏果蝇转座元件将基因导入地中海实蝇（Ceratitis capitata）。
Science. 1995 Dec 22;270(5244):2002-5. doi: 10.1126/science.270.5244.2002.
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The white gene of Ceratitis capitata: a phenotypic marker for germline transformation.地中海实蝇的白色基因：种系转化的表型标记
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