Harikumar P, Ninjoor V
Biochim Biophys Acta. 1979 Sep 20;587(1):60-6. doi: 10.1016/0304-4165(79)90220-4.
Starvation-induced alterations in liver lysosomes and their recovery pattern following refeeding were investigated. Fasting of adult rats for five days caused an increase in 'free' activities of acid hydrolyses in liver homogenates and loss in sedimentation of one of the heterogenous populations of lysosomes that could be isolated by differential centrifugation. Isopycnic sucrose gradient centrifugation revealed a decrease in the median and modal equilibration densities of all the forms of lysosomes in response to the dietary deprivation. Further, starvation also evoked a distinct bimodal distribution in a population that was rich in acid phosphatases, beta-galactosidase and N-acetyl-beta-glucosaminidase. Realimentation of starved animals for 10 days was found to restore the enzyme levels and the sedimentation characteristics to normal profiles.
研究了饥饿诱导的肝脏溶酶体变化及其再喂养后的恢复模式。成年大鼠禁食五天导致肝脏匀浆中酸性水解酶“游离”活性增加，以及通过差速离心可分离的一种异质性溶酶体群体沉降能力丧失。等密度蔗糖梯度离心显示，饮食剥夺导致所有形式的溶酶体的中位和模态平衡密度降低。此外，饥饿还在富含酸性磷酸酶、β-半乳糖苷酶和N-乙酰-β-葡萄糖胺酶的群体中引发了明显的双峰分布。发现饥饿动物再喂养10天可使酶水平和沉降特征恢复到正常状态。
