Wójczyk B, Lityńska A, Kalisz A
Laboratory for Biostructural Research, Jagiellonian University, Kraków, Poland.
Chronobiol Int. 1986;3(1):29-37. doi: 10.3109/07420528609083157.
In this paper circadian changes in the liver enzyme activities of rat housed under highly standardized conditions with 12: 12 hour light-dark cycle are shown. Activities of acid phosphatase, arylsulphatase, beta-galactosidase and beta-N-acetyl-D-glucosaminidase in microsomal and lysosomal fractions and crude homogenate were estimated every 4 hr during one 24-hr period. The enzyme activities were related to 1 mg of protein, 1 mg of DNA and 1 g fresh tissue. Daily changes of enzyme activities were found. In case of activity calculated per 1 mg DNA two maxima at 0500 and at 2100 hr were observed, while activity calculated per 1 mg protein showed one maximum at 0500 hr. Activity calculated per 1 g fresh tissue showed the maximum at 0500 hr for each enzyme only in microsomal fraction. As far as acrophase table is concerned for all enzymes and fractions the acrophase occurred during the night. The obtained results are discussed in relation to lysosomal enzymes synthesis process as well as different reference values.
本文展示了在高度标准化条件下饲养的大鼠，其肝脏酶活性的昼夜变化情况，这些大鼠处于12:12小时明暗循环中。在一个24小时的时间段内，每4小时对微粒体、溶酶体组分和粗匀浆中的酸性磷酸酶、芳基硫酸酯酶、β-半乳糖苷酶和β-N-乙酰-D-葡萄糖胺酶的活性进行评估。酶活性以每1毫克蛋白质、每1毫克DNA和每1克新鲜组织为单位进行计算。发现了酶活性的每日变化。以每1毫克DNA计算活性时，在05:00和21:00时观察到两个最大值，而以每1毫克蛋白质计算活性时，在05:00时出现一个最大值。仅在微粒体组分中，以每1克新鲜组织计算活性时，每种酶在05:00时出现最大值。就所有酶和组分的峰值相位表而言，峰值相位出现在夜间。结合溶酶体酶合成过程以及不同的参考值对所得结果进行了讨论。
