Della Ragione F, Criniti V, Della Pietra V, Borriello A, Oliva A, Indaco S, Yamamoto T, Zappia V
Department of Biochemistry and Biophysics F. Cedrangolo, Medical School, Second University of Naples, Italy.
FEBS Lett. 2001 Jun 22;499(3):199-204. doi: 10.1016/s0014-5793(01)02539-x.
A wealth of evidence correlates the chemopreventive activity of a fiber-rich diet with the production of butyrate. In order to identify the genes transcriptionally modulated by the molecule, we analyzed the expression profile of butyrate-treated colon cancer cells by means of cDNA expression arrays. Moreover, the effect of trichostatin A, a specific histone deacetylase inhibitor, was studied. A superimposable group of 23 genes out of 588 investigated is modulated by both butyrate and trichostatin A. Among them, a major target was tob-1, a gene involved in the control of cell cycle. tob-1 is also up-regulated by butyrate in a neuroblastoma-derived cell line, and its overexpression in the colon cells caused growth arrest. Our findings represent an extensive analysis of genes modulated by butyrate and identify completely new effectors of its biological activities.
大量证据表明，富含纤维的饮食的化学预防活性与丁酸盐的产生相关。为了鉴定受该分子转录调控的基因，我们通过cDNA表达阵列分析了丁酸盐处理的结肠癌细胞的表达谱。此外，还研究了特异性组蛋白脱乙酰酶抑制剂曲古抑菌素A的作用。在588个研究基因中，有23个基因组成的一个可叠加组受丁酸盐和曲古抑菌素A共同调控。其中，一个主要靶点是tob-1，一个参与细胞周期调控的基因。tob-1在成神经细胞瘤衍生的细胞系中也被丁酸盐上调，其在结肠细胞中的过表达导致生长停滞。我们的研究结果代表了对受丁酸盐调控的基因的广泛分析，并鉴定出了其生物活性的全新效应因子。
