将经皮附睾精子抽吸术（PESA）获取的精子卵胞浆内单精子注射（ICSI）到体外成熟卵母细胞后发生的双胎持续妊娠：病例报告

Ongoing twin pregnancy after ICSI of PESA-retrieved spermatozoa into in-vitro matured oocytes: case report.

作者信息

Abdul-Jalil A K, Child T J, Phillips S, Dean N, Carrier S, Tan S L

机构信息

Department of Obstetrics and Gynecology, McGill Reproductive Center, Royal Victoria Hospital F6.58, McGill University, 687 Pine Avenue West, Montreal, Quebec, H3A 1A1, Canada.

出版信息

Hum Reprod. 2001 Jul;16(7):1424-6. doi: 10.1093/humrep/16.7.1424.

DOI:10.1093/humrep/16.7.1424

PMID:11425824

Abstract

The recovery of immature oocytes from unstimulated ovaries followed by in-vitro maturation (IVM) is an attractive alternative to conventional IVF in the treatment of female infertility. Similarly, surgical recovery of spermatozoa from the epididymis by percutaneous sperm aspiration (PESA) has simplified the retrieval of the male gamete in treatment of men with obstructive azoospermia. We report the first ongoing clinical twin pregnancy resulting from intracytoplasmic sperm injection (ICSI) of spermatozoa retrieved by PESA into IVM oocytes. In the treatment of a 24-year old woman, 12 immature oocytes were retrieved. Six oocytes matured (maturation rate 50%) after 24-hour incubation and were inseminated by ICSI. Four oocytes had two pronuclei (fertilization rate 67%) and 3 good quality embryos were transferred. A viable twin pregnancy was confirmed by ultrasound scan. This report illustrates the use of a combination of less invasive assisted reproductive techniques in overcoming barriers to infertility.

摘要

从未经刺激的卵巢中获取未成熟卵母细胞，随后进行体外成熟（IVM），这是治疗女性不孕症时一种有吸引力的替代传统体外受精（IVF）的方法。同样，通过经皮附睾精子抽吸术（PESA）从附睾中手术回收精子，简化了梗阻性无精子症男性患者配子的获取过程。我们报告了首例通过PESA获取的精子卵胞浆内单精子注射（ICSI）到IVM卵母细胞后正在进行的临床双胎妊娠。在治疗一名24岁女性时，获取了12个未成熟卵母细胞。24小时孵育后，6个卵母细胞成熟（成熟率50%），并通过ICSI进行授精。4个卵母细胞有两个原核（受精率67%），移植了3个优质胚胎。超声扫描确认了存活的双胎妊娠。本报告说明了使用侵入性较小的辅助生殖技术组合来克服不孕症障碍的情况。