Nemcovie M, Farkas V
Institute of Chemistry, Slovak Academy of Sciences, Bratislava.
Acta Biol Hung. 2001;52(2-3):281-8. doi: 10.1556/ABiol.52.2001.2-3.12.
The differentiation and metabolism in the soil-borne saprophytic deuteromycete Trichoderma viride are subject to control by light. We have investigated the effect of illumination of mycelia on the activities of cell-wall synthesizing enzymes beta-1,3-glucan synthase and chitin synthase and on the composition of cell alls. After 10 min illumination of dark-grown mycelia with white fluorescent light at 600 1x, a gradual rise in specific activity of membrane-bound enzymes beta-1,3-glucan synthase by about 130% and a decrease in specific activity of chitin synthase by about 50% in relation to the dark control were observed. The changes in enzyme activities were caused by de novo synthesis of corresponding polysaccharide synthases(s) and/or their regulatory components since they were not observed when protein synthesis was blocked with 50 microg/ml cycloheximide. The content of beta-1,3-glucan in the cell walls of illuminated mycelia has increased by 35-50% in comparison to the dark control while the content of chitin remained practically unchanged.
土壤腐生型半知菌绿色木霉的分化和代谢受光照控制。我们研究了菌丝体光照对细胞壁合成酶β-1,3-葡聚糖合酶和几丁质合酶活性以及细胞壁组成的影响。用600勒克斯的白色荧光灯对黑暗中生长的菌丝体光照10分钟后，与黑暗对照相比，膜结合酶β-1,3-葡聚糖合酶的比活性逐渐升高约130%，几丁质合酶的比活性降低约50%。酶活性的变化是由相应多糖合酶和/或其调节成分的从头合成引起的，因为当用50微克/毫升放线菌酮阻断蛋白质合成时未观察到这些变化。与黑暗对照相比，光照菌丝体细胞壁中β-1,3-葡聚糖的含量增加了35 - 50%，而几丁质的含量实际上保持不变。
