牛红细胞膜中独特的磷脂组织。
A unique phospholipid organization in bovine erythrocyte membranes.
作者信息
Florin-Christensen J, Suarez C E, Florin-Christensen M, Wainszelbaum M, Brown W C, McElwain T F, Palmer G H
机构信息
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Jul 3;98(14):7736-41. doi: 10.1073/pnas.131580998. Epub 2001 Jun 26.
Abstract
Ruminant erythrocytes are remarkable for their choline-phospholipid anomalies; namely, low or absent phosphatidylcholine (PC) along with high sphingomyelin levels. Here, we report another anomaly in bovine erythrocytes that affects aminophospholipids: phosphatidylethanolamine (PE) shows an extreme asymmetry, with only 2% of the total present in the outer leaflet. Furthermore, we found that phospholipase A(2), an enzyme located on the external surface of the erythrocytes, shows higher activity against PC than against PE. In addition, we observed that acylation of PE is by far the most important biosynthetic event in this system. We propose that deacylation of PE and PC by phospholipase A(2) to generate lysocompounds, followed by selective reacylation of lyso-PE in the inner leaflet, can account for the compositional and architectural peculiarities of bovine erythrocyte membranes.
摘要
反刍动物红细胞因其胆碱磷脂异常而引人注目；即磷脂酰胆碱（PC）含量低或缺乏，同时鞘磷脂水平高。在此，我们报告牛红细胞中另一种影响氨基磷脂的异常情况：磷脂酰乙醇胺（PE）表现出极端的不对称性，在外膜层中仅占总量的2%。此外，我们发现位于红细胞外表面的磷脂酶A2对PC的活性高于对PE的活性。另外，我们观察到PE的酰化是该系统中迄今为止最重要的生物合成事件。我们提出，磷脂酶A2对PE和PC进行脱酰基作用以生成溶血化合物，随后在内膜层对溶血PE进行选择性再酰化，这可以解释牛红细胞膜的组成和结构特性。
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