Ledwozyw A, Stolarczyk H
Department of Pathophysiology, Veterinary Faculty, Agricultural Academy, Lublin, Poland.
Acta Vet Hung. 1991;39(3-4):187-95.
Phospholipid topology in erythrocyte membranes of bronchopneumonic calves and changes in phospholipid asymmetry induced by incubation of erythrocytes with neutrophils of diseased calves were examined. Using aminophospholipid tracing by non-penetrating probe, trinitrobenzenesulfonic acid, and phospholipid hydrolysis by specific phospholipase A2 from Naja naja venom and sphingomyelinase from Staphylococcus aureus it was stated that in erythrocytes from diseased calves phosphatidylserine and, to some extent, phosphatidylethanolamine are externalized on the outer leaflet of the membrane. Similar results were obtained for erythrocytes of healthy calves after incubation with granulocytes of bronchopneumonic animals. It is suggested that granulocytes of bronchopneumonic calves cause perturbations in erythrocyte membrane phospholipid organization.
研究了支气管肺炎犊牛红细胞膜中的磷脂拓扑结构，以及患病犊牛的红细胞与中性粒细胞孵育后诱导的磷脂不对称性变化。使用非穿透性探针三硝基苯磺酸进行氨基磷脂追踪，以及使用眼镜蛇毒中的特异性磷脂酶A2和金黄色葡萄球菌中的鞘磷脂酶进行磷脂水解，结果表明，患病犊牛红细胞中的磷脂酰丝氨酸以及在一定程度上的磷脂酰乙醇胺在外膜小叶上被外化。健康犊牛的红细胞与支气管肺炎动物的粒细胞孵育后也得到了类似结果。提示支气管肺炎犊牛的粒细胞会引起红细胞膜磷脂组织的紊乱。
