Smyth R W, Kahlmeter G, Olsson Liljequist B, Hoffman B
Department of Microbiology, Central Hospital, Växjö, SE-351 85, Sweden.
J Hosp Infect. 2001 Jun;48(2):103-7. doi: 10.1053/jhin.2001.0933.
Five methods for the detection of methicillin-resistant Staphylococcus aureus were used to examine a collection of 100 clinical isolates comprising both susceptible and resistant strains. The disc diffusion test with oxacillin had a sensitivity of 93.3% and a specificity of 92.0% whereas mannitol salt agar containing oxacillin had a sensitivity of 100% and specificity 80.6% with high inoculum. With a low inoculum the sensitivity was 90.7% and specificity 96.0%. The MRSA screen test (Denka Seiken Co. Ltd., Japan) and Evigene MRSA Detection Kit (State Serum Institute, Denmark) tests were in complete agreement with results obtained with polymerase chain reaction assays amplifying mecA and nuc gene sequences.
采用五种检测耐甲氧西林金黄色葡萄球菌的方法，对100株临床分离株（包括敏感株和耐药株）进行检测。用苯唑西林纸片扩散试验的灵敏度为93.3%，特异性为92.0%，而含苯唑西林的甘露醇盐琼脂在高接种量时灵敏度为100%，特异性为80.6%。低接种量时，灵敏度为90.7%，特异性为96.0%。MRSA筛选试验（日本Denka Seiken公司）和Evigene MRSA检测试剂盒（丹麦国家血清研究所）的检测结果与通过扩增mecA和nuc基因序列的聚合酶链反应分析所获得的结果完全一致。
