Yoshida Y, Hosoda E, Nakamura T, Yamamoto T
Department of Oncology, The Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Jpn J Cancer Res. 2001 Jun;92(6):592-6. doi: 10.1111/j.1349-7006.2001.tb01135.x.
A 35-kDa protein, ANA, belongs to an emerging family of antiproliferative proteins consisting of Tob, Tob2, ANA / BTG3, PC3B, PC3 / TIS21 / BTG2, and BTG1. All of these, except ANA and PC3B, have been shown to interact with the CCR4 transcription factor-associated protein Caf1. Here we show that ANA also associates with Caf1, ANA being the preferred partner of Caf1 among the Tob family proteins. Although ANA is likely to interact with Caf1 at its amino-terminal half, which is conserved among the family members, our data suggest that the carboxyl-terminal half of ANA plays a role in the interaction. Finally, in situ hybridization experiments revealed that expression of Caf1 overlaps at least in part with that of ANA. Thus, ANA could function through its interaction with Caf1.
一种35千道尔顿的蛋白质ANA，属于一个新出现的抗增殖蛋白家族，该家族由Tob、Tob2、ANA / BTG3、PC3B、PC3 / TIS21 / BTG2和BTG1组成。除了ANA和PC3B之外，所有这些蛋白都已被证明能与CCR4转录因子相关蛋白Caf1相互作用。在此我们表明，ANA也与Caf1相关联，在Tob家族蛋白中，ANA是Caf1的首选伙伴。尽管ANA可能在其氨基末端一半区域与Caf1相互作用，这在家族成员中是保守的，但我们的数据表明，ANA的羧基末端一半区域在这种相互作用中发挥作用。最后，原位杂交实验显示，Caf1的表达至少部分与ANA的表达重叠。因此，ANA可能通过与Caf1的相互作用发挥功能。
