Liu Lingqi, Liu Shuchao, Duan Qixin, Chen Liang, Wu Tianpeng, Qian Huijun, Yang Sixing, Xin Dianqi, He Zhisong, Guo Yinglu
Department of Urology, Renmin Hospital of Wuhan UniversityWuhan 430060, China.
Department of Urology, Peking University, First HospitalBeijing 100034, China.
Am J Transl Res. 2017 May 15;9(5):2394-2402. eCollection 2017.
Some microRNA (miRNA) levels have been found to be dysregulated in cancer patients, suggesting the potential usefulness of miRNAs in cancer therapies. The purpose of this study was to investigate the expression of miR-142-5p in human renal cell carcinoma (RCC) and its potential role in tumor growth and metastasis.
The expression level of miR-142-5p in human RCC tissue and cell lines was determined by quantitative reverse transcription polymerase chain reaction analysis. MTT, colony formation, Transwell, and cell cycle assays were performed to explore the potential functions of miR-142-5p in human RCC cells. The potential target gene of miR-142-5p was identified and confirmed via luciferase reporter assays.
miR-142-5p expression was elevated in RCC tissues and cell lines. Overexpression of miR-142-5p significantly promoted cell proliferation and colony formation and could prevent G1 phase arrest among RCC 786-O cells. Meanwhile, the migration potential of 786-O cells was greater than that of control cells. BTG3 was identified as a direct target of miR-142-5p, and re-expression of BTG3 reversed the miR-142-5p-induced cell proliferation.
miR-142-5p promoted the proliferation and migration of RCC cells by targeting BTG3. With this potential onco-miRNA role in the progression of RCC, miR-142-5p may be a therapeutic target for the treatment of RCC.
已发现癌症患者体内一些微小RNA（miRNA）水平失调，这表明miRNA在癌症治疗中具有潜在应用价值。本研究旨在探讨miR-142-5p在人肾细胞癌（RCC）中的表达及其在肿瘤生长和转移中的潜在作用。
采用定量逆转录聚合酶链反应分析检测人RCC组织和细胞系中miR-142-5p的表达水平。进行MTT、集落形成、Transwell和细胞周期分析，以探索miR-142-5p在人RCC细胞中的潜在功能。通过荧光素酶报告基因分析鉴定并确认miR-142-5p的潜在靶基因。
miR-142-5p在RCC组织和细胞系中表达升高。miR-142-5p的过表达显著促进细胞增殖和集落形成，并可防止RCC 786-O细胞发生G1期阻滞。同时，786-O细胞的迁移能力强于对照细胞。BTG3被鉴定为miR-142-5p的直接靶标，BTG3的重新表达逆转了miR-142-5p诱导的细胞增殖。
miR-142-5p通过靶向BTG3促进RCC细胞的增殖和迁移。鉴于其在RCC进展中具有这种潜在的致癌miRNA作用，miR-142-5p可能成为RCC治疗的一个靶点。
