Lee M J, Gergely F, Jeffers K, Peak-Chew S Y, Raff J W
Department of Genetics, Wellcome/CRC Institute, Tennis Court Road, Cambridge CB2 1QR, UK.
Nat Cell Biol. 2001 Jul;3(7):643-9. doi: 10.1038/35083033.
The XMAP215/ch-TOG/Msps family of microtubule-associated proteins (MAPs) promote microtubule growth in vitro and are concentrated at centrosomes in vivo. We show here that Msps (mini-spindles protein) interacts with the centrosomal protein D-TACC, and that this interaction strongly influences microtubule behaviour in Drosophila embryos. If D-TACC levels are reduced, Msps does not concentrate at the centrosomes efficiently and the centrosomal microtubules appear to be destabilized. If D-TACC levels are increased, both D-TACC and Msps accumulate around the centrosomes/spindle poles, and the centrosomal microtubules appear to be stabilized. We show that the interaction between D-TACC and Msps is evolutionarily conserved. We propose that D-TACC and Msps normally cooperate to stabilize centrosomal microtubules by binding to their minus ends and binding to their plus ends as they grow out from the centrosome.
微管相关蛋白（MAPs）的XMAP215/ch-TOG/Msps家族在体外可促进微管生长，在体内则集中于中心体。我们在此表明，Msps（微纺锤体蛋白）与中心体蛋白D-TACC相互作用，且这种相互作用对果蝇胚胎中的微管行为有强烈影响。如果D-TACC水平降低，Msps无法有效集中于中心体，中心体微管似乎会不稳定。如果D-TACC水平升高，D-TACC和Msps都会在中心体/纺锤体极周围积聚，中心体微管似乎会稳定。我们表明，D-TACC与Msps之间的相互作用在进化上是保守的。我们提出，D-TACC和Msps通常通过结合微管负端并在微管从中心体长出时结合其正端来共同稳定中心体微管。
