Kumar R K, Yang Z, Bilson S, Thliveris S, Cooke B E, Geczy C L
School of Pathology, University of New South Wales, Sydney, Australia.
J Leukoc Biol. 2001 Jul;70(1):59-64.
S100A8 is a major cytoplasmic protein of neutrophils and monocytes/macrophages and has been associated with myeloid cell differentiation and activation. Little is known about its functions or mechanisms of release from neutrophils. We have developed a monoclonal antibody to murine S100A8, which cross-reacts with human S100A8. This antibody, which recognizes the homodimeric form of the protein, detects its expression specifically in human neutrophils and is reactive in formalin-fixed, paraffin-embedded tissues. Using this antibody as well as a commercially available antibody to human S100A8, we show that phagocytic activation of neutrophils, in vivo in acute appendicitis and in vitro following phagocytosis of opsonized zymosan, is characterized by loss of cytoplasmic immunoreactivity for S100A8. In vitro, phagocytosis is associated with rapid diminution of immunostaining without loss of viability. Loss of immunoreactivity for S100A8 may serve as a marker of localized neutrophil activation in tissues.
S100A8是中性粒细胞和单核细胞/巨噬细胞的一种主要胞质蛋白，与髓样细胞分化和激活有关。关于其功能或从中性粒细胞释放的机制知之甚少。我们已经开发出一种针对小鼠S100A8的单克隆抗体，该抗体可与人S100A8发生交叉反应。这种识别该蛋白同二聚体形式的抗体，特异性检测到其在人中性粒细胞中的表达，并且在福尔马林固定、石蜡包埋的组织中具有反应性。使用这种抗体以及一种市售的抗人S100A8抗体，我们发现，在急性阑尾炎的体内以及在体外吞噬调理酵母聚糖后，中性粒细胞的吞噬激活表现为S100A8胞质免疫反应性丧失。在体外，吞噬作用与免疫染色的快速减弱有关，但细胞活力并未丧失。S100A8免疫反应性丧失可能是组织中局部中性粒细胞激活的一个标志。
