Hosobe H
Department of Pediatrics, Tokyo Medical University.
Arerugi. 2001 May;50(5):450-6.
The lymphocyte membrane fluidity of patients with allergic diseases was measured by electron spin resonance (ESR), and the effect of the epinephrine stimulation on the membrane fluidity was examined. The peripheral lymphocytes were obtained from 15 patients with bronchial asthma and atopic dermatitis (28.7 +/- 9.9 years old, 10 females and 5 males) and 11 healthy adults (30.5 +/- 4.6 years old, 2 females and 9 males). Lymphocyte membranes were spin-labeled with 5-doxyl-stearic acid. Before and after the stimulation of epinephrine of the final concentrations at 10(-5) and 10(-4) mol/l, ESR spectra of the outer membranes were analyzed to evaluate the membrane fluidity. The membrane fluidity of the intact lymphocytes of allergic patients was significantly decreased in comparison to healthy controls. Although the epinephrine stimulation increased the lymphocyte membrane fluidity, the increase in fluidity was less in allergic patients than in healthy controls. There are various receptors on the surface of the lymphocyte membranes, and changes of the membrane fluidity have an influence on their functions. The results in this study elucidate the decreased fluidity of lymphocyte membrane in patients with bronchial asthma and atopic dermatitis, and suggest that the functions of the membrane receptors might be impaired.
采用电子自旋共振（ESR）技术测定过敏性疾病患者淋巴细胞膜流动性，并检测肾上腺素刺激对膜流动性的影响。从15例支气管哮喘和特应性皮炎患者（年龄28.7±9.9岁，女性10例，男性5例）和11名健康成年人（年龄30.5±4.6岁，女性2例，男性9例）获取外周血淋巴细胞。用5 - 硬脂酰基 - 二茂铁甲酸对淋巴细胞膜进行自旋标记。在终浓度为10(-5)和10(-4)mol/L的肾上腺素刺激前后，分析外膜的ESR光谱以评估膜流动性。与健康对照相比，过敏性患者完整淋巴细胞的膜流动性显著降低。尽管肾上腺素刺激增加了淋巴细胞膜流动性，但过敏性患者的流动性增加幅度小于健康对照。淋巴细胞膜表面存在多种受体，膜流动性的变化会影响其功能。本研究结果阐明了支气管哮喘和特应性皮炎患者淋巴细胞膜流动性降低，并提示膜受体功能可能受损。
