Antúnez Cristina, Torres María Jose, Mayorga Cristobalina, Corzo José L, Jurado Antonio, Santamaría-Babi Luis F, Vera Angel, Blanca Miguel
Research Unit for Allergic Diseases, Allergic Service, Carlos Haya Hospital, Málaga, Spain.
Pediatr Allergy Immunol. 2006 May;17(3):166-74. doi: 10.1111/j.1399-3038.2006.00390.x.
T cells are known to develop a critical role in the pathogenesis of atopic dermatitis (AD) and bronchial asthma. T cells involved in AD express the skin homing receptor CLA, but no lung homing receptor has been identified in bronchial asthma. We compared different cell markers and the cytokine production in T cells from children with AD or bronchial asthma. We studied the involvement of CLA+ and CLA- T-cell subpopulations in these diseases. We studied 20 children with acute AD lesions, 15 with mild persistent asthma, and 15 non-atopic controls. All patients were sensitized to house dust mite (DP) and evaluated during the acute phase. Total and specific IgE were measured by immunoassay and the expression of different cell markers and the cytokine production was analyzed by flow cytometry in peripheral blood mononuclear cells. Total IgE was significantly higher in AD children and IgE to DP in the asthmatic children. There was a significant increase in CD25+ CD4+ cells in asthmatic children and in HLA-DR+ CD4+ and HLA-DR+ CD8+ cells in AD. In the CD4+ subsets, there was an increase in IL-13, IL-5 and TNF-alpha in AD compared to controls, a decrease in IFN-gamma in asthmatic children compared to controls, and an increase in IL-13, IL5, IL2, TNF-alpha, and IFN-gamma in the AD compared to asthmatic children. Changes in cytokine production were mainly detected in CLA+ cells in AD and in CLA- cells in asthma. Differences exist in total and specific IgE, activation markers, and cytokine patterns between AD children and children with asthma, with the former expressing a Th2 pattern whereas in asthmatic children we only detected a decrease in IFN-gamma. Moreover, the subpopulations (CLA+ vs. CLA-) expressing these changes were different, indicating that the underlying mechanisms in the two diseases are not exactly the same.
已知T细胞在特应性皮炎（AD）和支气管哮喘的发病机制中发挥关键作用。参与AD的T细胞表达皮肤归巢受体CLA，但在支气管哮喘中尚未发现肺归巢受体。我们比较了AD或支气管哮喘患儿T细胞中的不同细胞标志物和细胞因子产生情况。我们研究了CLA +和CLA-T细胞亚群在这些疾病中的作用。我们研究了20例患有急性AD皮损的儿童、15例轻度持续性哮喘儿童和15例非特应性对照。所有患者均对屋尘螨（DP）致敏，并在急性期进行评估。通过免疫测定法测量总IgE和特异性IgE，并通过流式细胞术分析外周血单核细胞中不同细胞标志物的表达和细胞因子产生情况。AD患儿的总IgE显著更高，哮喘患儿的DP特异性IgE更高。哮喘患儿的CD25 + CD4 +细胞以及AD患儿的HLA-DR + CD4 +和HLA-DR + CD8 +细胞显著增加。在CD4 +亚群中，与对照组相比，AD患儿的IL-13、IL-5和TNF-α增加，哮喘患儿的IFN-γ与对照组相比减少，与哮喘患儿相比，AD患儿的IL-13、IL5、IL2、TNF-α和IFN-γ增加。细胞因子产生的变化主要在AD的CLA +细胞和哮喘的CLA-细胞中检测到。AD患儿和哮喘患儿在总IgE和特异性IgE、激活标志物及细胞因子模式方面存在差异，前者表现为Th2模式，而在哮喘患儿中我们仅检测到IFN-γ减少。此外，表达这些变化的亚群（CLA +与CLA-）不同，表明这两种疾病的潜在机制并不完全相同。
