He H, Lu X, Yang Y
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2001 Jun;15(2):120-4.
To determine the classification, phylogenetic and genetic type of XJ-90260 and XJ-91006 viruses isolated in China.
Two pairs of specific primers were designed to amplify the NSP4 and E1-3' UTR genes of XJ-90260 and XJ-91006 viruses and the nucleotide sequences between them and with that of other Alphavirus were compared.
The nucleotide sequences identity between XJ-90260 and XJ-91006 viruses was 100% and has showed the highest with WEE, comparing with the other 26 Alphaviruses in 3'-UTR. NSP4 gene of XJ-90260 and XJ-91006 viruses high homologous with EEE virus and E-1 gene high homologous wih SIN virus. XJ-90260 and XJ-91006 viruses belong to the B group of WEE and are closest to the Russian strain (Y62-33) by phylogenetic analysis.
XJ-90260 and XJ-91006 viruses isolated in China are recombinant Alphaviruses, they belong to the same genetic type as WEE does.
确定在中国分离出的XJ - 90260和XJ - 91006病毒的分类、系统发育和基因类型。
设计两对特异性引物，扩增XJ - 90260和XJ - 91006病毒的NSP4和E1 - 3'UTR基因及其之间的核苷酸序列，并与其他甲病毒的序列进行比较。
XJ - 90260和XJ - 91006病毒之间的核苷酸序列同一性为100%，在3'-UTR中与西部马脑炎病毒（WEE）的同一性最高，与其他26种甲病毒相比。XJ - 90260和XJ - 91006病毒的NSP4基因与东部马脑炎病毒（EEE）高度同源，E - 1基因与辛德毕斯病毒（SIN）高度同源。通过系统发育分析，XJ - 90260和XJ - 91006病毒属于WEE的B组，与俄罗斯毒株（Y62 - 33）关系最密切。
在中国分离出的XJ - 90260和XJ - 91006病毒是重组甲病毒，它们与WEE属于同一基因类型。
