Asano Y, Watanabe S
Biotechnology Research Center, Toyama Prefectural University, Kosugi, Japan.
Biosci Biotechnol Biochem. 2001 May;65(5):1191-4. doi: 10.1271/bbb.65.1191.
Microbial degraders of poly(3-hydroxybutyrate) (PHB) were isolated from soil. Arthrobacter sp. strain W6 used not only PHB as a carbon source, but also PHAs such as poly(3-hydroxybutyrate-co-[5%]3-hydroxyvalerate), poly(3-hydroxybutyrate-co-[14%]3-hydroxyvalerate), and poly(3-hydroxybutyrate-co-[22%]3-hydroxyvalerate). PHB-depolymerase was purified to homogeneity from the culture broth of Arthrobacter sp. strain W6 by a procedure involving DEAE- and butyl-Toyopearl column chromatographies. The Mr of the enzyme was estimated to be about 47,000 by SDS-polyacrylamide gel electrophoresis. The enzyme was most active at pH 8.5 and 50 degrees C, and was inhibited by phenylmethylsulfonyl fluoride, Hg2+, Ag+, and Pb2+.
从土壤中分离出聚（3-羟基丁酸酯）（PHB）的微生物降解菌。节杆菌属菌株W6不仅利用PHB作为碳源，还利用聚羟基脂肪酸酯，如聚（3-羟基丁酸酯-co-[5%]3-羟基戊酸酯）、聚（3-羟基丁酸酯-co-[14%]3-羟基戊酸酯）和聚（3-羟基丁酸酯-co-[22%]3-羟基戊酸酯）。通过涉及DEAE和丁基- Toyopearl柱色谱的方法，从节杆菌属菌株W6的培养液中纯化出PHB解聚酶至均一性。通过SDS-聚丙烯酰胺凝胶电泳估计该酶的分子量约为47,000。该酶在pH 8.5和50℃时活性最高，并且受到苯甲基磺酰氟、Hg2 +、Ag +和Pb2 +的抑制。
