Ueta H, Maeda Y, Kawai Y
College of Science and Engineering, Iwaki Meisei University, Fukushima, Japan.
Biosci Biotechnol Biochem. 2001 May;65(5):1261-4. doi: 10.1271/bbb.65.1261.
The interaction of distamycin with ColE1 DNA was examined by using differential scanning calorimetry (DSC) taking the helix-coil transition theory of DNA into consideration. Our results here strongly indicate that the affinity of distamycin to DNA, at a low distamycin concentration, depends highly on the DNA sequence, and preferential binding occurs to the sites of four to six successive A-T pairs having two or more successive G-C pairs on both their ends.
考虑到DNA的螺旋-卷曲转变理论，采用差示扫描量热法（DSC）研究了偏端霉素与ColE1 DNA的相互作用。我们的研究结果有力地表明，在低偏端霉素浓度下，偏端霉素对DNA的亲和力高度依赖于DNA序列，并且优先结合于两端具有两个或更多连续G-C对的四至六个连续A-T对的位点。
