Grokhovsky S L, Surovaya A N, Burckhardt G, Pismensky V F, Chernov B K, Zimmer C, Gursky G V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
FEBS Lett. 1998 Nov 20;439(3):346-50. doi: 10.1016/s0014-5793(98)01379-9.
We studied the interaction of cis-diammine Pt(II)-bridged bis-netropsin, cis-diammine Pt(II)-bridged bis-distamycin and oligomethylene-bridged bis-netropsin with synthetic DNA fragments containing pseudosymmetrical AT-rich nucleotide sequences and compared it with the interaction of the parent compounds netropsin and distamycin A. For fragments containing multiple blocks of (AIT)4 and (T/A)4 separated by zero, one, two and three GC-base pairs, DNase I footprinting and CD spectroscopy studies reveal that 5'-TTTTAAAA-3' is the strongest affinity binding site for cis-diammine Pt(II)-bridged bis-netropsin and bis-distamycin. They both bind less strongly to a DNA region containing the sequence 5'-AAAATTTT-3'. Netropsin, distamycin A and oligomethylene-bridged bis-netropsin exhibit far less sequence discrimination.
我们研究了顺式二氨合铂（II）桥联双奈替普辛、顺式二氨合铂（II）桥联双偏端霉素和亚甲基桥联双奈替普辛与含有假对称富含AT核苷酸序列的合成DNA片段的相互作用，并将其与母体化合物奈替普辛和偏端霉素A的相互作用进行了比较。对于含有多个由零、一、二和三个GC碱基对分隔的（AIT）4和（T/A）4片段，DNase I足迹法和圆二色光谱研究表明，5'-TTTTAAAA-3'是顺式二氨合铂（II）桥联双奈替普辛和双偏端霉素的最强亲和力结合位点。它们与含有5'-AAAATTTT-3'序列的DNA区域的结合较弱。奈替普辛、偏端霉素A和亚甲基桥联双奈替普辛表现出的序列选择性要少得多。
