Bicka L, Kuźmak J, Kozaczyńska B, Plucienniczak A, Skorupska A
Department of Biochemistry, National Veterinary Research Institute, Puławy, Poland.
Acta Biochim Pol. 2001;48(1):227-32.
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and expressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immunoblotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for detection of BLV antibodies in the infected cattle.
牛白血病病毒（BLV）的gag基因编码蛋白p24在大肠杆菌中被克隆并表达为硫氧还蛋白-6xHis-p24蛋白。携带质粒pT7THis-p24的细菌细胞表达了38 kDa的蛋白，该蛋白通过使用抗p24单克隆抗体以及来自感染BLV的牛和羊的血清进行免疫印迹分析得以检测。纯化的p24融合蛋白在检测感染牛体内的BLV抗体时表现出敏感性和特异性。
