核糖体依赖的含多聚腺苷酸的不均一核RNA向较小RNA分子的转化。
Ribosome-dependent conversion of polyA-containing heterogenous nuclear RNA into smaller RNA molecules.
作者信息
Grozdanovic J, Hradec J
出版信息
Nucleic Acids Res. 1975 Jun;2(6):821-30. doi: 10.1093/nar/2.6.821.
Abstract
The polyA-containing heterogenous nuclear RNA fraction separated from total rat liver nRNA by gel filtration on Sepharose 4B followed by affinity chromatography on polyU-Sepharose and containing predominantly the 45S components becomes enzymatically bound to homologous 80S ribosomes and polyribosomes at 0 degree C. If 80S ribosomes or polyribosomes with bound poly-a-containing HnRNA are subjected to a further incubation at 37 degree C, the original 45S RNA is gradually converted into smaller RNA species of 10- 35S which remain bound to the particle. This ribosome-dependent cleavage of larger HnRNA species into smaller RNA molecules may represent the ultimate step of mRNA maturation.
摘要
通过在琼脂糖4B上进行凝胶过滤，然后在聚尿苷酸 - 琼脂糖上进行亲和层析，从大鼠肝脏总核RNA中分离出的含多聚腺苷酸的不均一核RNA部分，主要包含45S组分，在0℃下酶促结合到同源的80S核糖体和多聚核糖体上。如果将结合有多聚腺苷酸的hnRNA的80S核糖体或多聚核糖体在37℃下进一步温育，原来的45S RNA会逐渐转化为10 - 35S的较小RNA种类，这些RNA仍与颗粒结合。这种核糖体依赖性的将较大的hnRNA种类切割成较小RNA分子的过程可能代表了mRNA成熟的最终步骤。
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