Wreschner D H, Herzberg M
Nucleic Acids Res. 1984 Feb 10;12(3):1349-59. doi: 10.1093/nar/12.3.1349.
A simple method has been developed that allows the rapid isolation and identification of highly resolved mRNA molecules. RNA species are separated by gel electrophoresis and then blotted on to a paper sheet to which polyuridylic acid has been covalently bound. This mRNA affinity paper ("mAP") specifically binds, in a reversible manner, polyA+ containing molecules. A replica picture of the agarose gel is thus obtained on the mAP, from which bound mRNA molecules can be eluted by heating in water. In addition to their simple isolation individual mRNA species, whilst still bound to mAP, can be identified by both "in-situ" hybridization and translation.
已开发出一种简单方法，可快速分离和鉴定高度分辨的mRNA分子。RNA种类通过凝胶电泳分离，然后印迹到共价结合了聚尿苷酸的纸片上。这种mRNA亲和纸（“mAP”）以可逆方式特异性结合含polyA +的分子。因此在mAP上获得了琼脂糖凝胶的复制图像，通过在水中加热可从该图像上洗脱结合的mRNA分子。除了简单分离单个mRNA种类外，仍结合在mAP上的单个mRNA种类还可通过“原位”杂交和翻译进行鉴定。
