Miyagi E, Katoh R, Li X, Lu S, Suzuki K, Maeda S, Shibuya M, Kawaoi A
Department of Pathology, Yamanashi Medical University, Nakakomagun, Japan.
Thyroid. 2001 Jun;11(6):539-43. doi: 10.1089/105072501750302831.
We studied the regulation of the expression of vascular endothelial growth factor (VEGF) by TSH in monolayer cultures of rat thyroid FRTL-5 cell lines. The VEGF mRNA synthesis was significantly inhibited by TSH as well as dibutyryl cyclic adenosine monophosphate (cAMP) in a dose-dependent manner in FRTL-5 cells. This observation is contrary to previously published results using the thyroid follicular culture system. Our results suggest that the direct effect of TSH/cAMP is to inhibit the VEGF synthesis in monolayer cells.
我们在大鼠甲状腺FRTL-5细胞系的单层培养物中研究了促甲状腺激素（TSH）对血管内皮生长因子（VEGF）表达的调节作用。在FRTL-5细胞中，TSH以及二丁酰环磷酸腺苷（cAMP）均以剂量依赖的方式显著抑制VEGF mRNA的合成。这一观察结果与先前使用甲状腺滤泡培养系统发表的结果相反。我们的结果表明，TSH/cAMP的直接作用是抑制单层细胞中的VEGF合成。
