通过析因法优化高产粘红酵母突变体32的类胡萝卜素产量。
Optimization of carotenoid production from hyper-producing Rhodotorula glutinis mutant 32 by a factorial approach.
作者信息
Bhosale P, Gadre R V
机构信息
Chemical Engineering Division, National Chemical Laboratory, Pune, India.
出版信息
Lett Appl Microbiol. 2001 Jul;33(1):12-6. doi: 10.1046/j.1472-765x.2001.00940.x.
AIMS
Optimization of carotenoid production by a mutant of Rhodotorula glutinis.
METHODS
The growth and carotenoid production was optimized in shake flasks using a two-level, three-variable factorial design.
RESULTS
The volumetric carotenoid production could be increased to 129 +/- 2 mg x 1(-1) in a medium containing (g x l(-1)) yeast extract 11.74, glucose 46 and threonine 18 along with other micronutrients, wherein, beta-carotene yield was 102 +/- 2 mg x l(-1), accounting for 80% of the total carotenoids.
SIGNIFICANCE AND IMPACT OF THE STUDY
The medium optimization resulted in a fourfold increase in volumetric production and a twofold increase in the cellular accumulation of carotenoids. In view of such high yields, the mutant of Rhodotorula glutinis can be a potential source of beta-carotene.
目的
优化粘红酵母突变体的类胡萝卜素产量。
方法
采用两水平、三变量析因设计在摇瓶中优化生长和类胡萝卜素产量。
结果
在含有（克/升）酵母提取物11.74、葡萄糖46和苏氨酸18以及其他微量营养素的培养基中，类胡萝卜素体积产量可提高到129±2毫克/升，其中β-胡萝卜素产量为102±2毫克/升，占总类胡萝卜素的80%。
研究的意义和影响
培养基优化使体积产量提高了四倍，类胡萝卜素的细胞积累增加了两倍。鉴于如此高的产量，粘红酵母突变体可能是β-胡萝卜素的潜在来源。
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