Vĕtvicka V, Viklický V, Zelený V
Folia Biol (Praha). 1979;25(3):156-68.
Some five to six per cent of mouse PEC spontaneously form rosettes with SRBC. This weak intercellular interaction is most likely mediated by a "receptor" for SRBC located on the surface of peritoneal macrophages. Manifestation of the receptor is influenced by the genetic background and the H-2 haplotype of PEC donors; a high proportion of RFC is associated with the H-2s haplotype. PEC derived from intact mice markedly differ in morphological and functional characteristics from those of PP- or TG-pretreated donors. Formation of rosettes by induced macrophages depends on the time interval between stimulation and PEC harvesting and on the type of the inducing agent; it is also radiosensitive and more responsive to the action of colchicine. TG-induced macrophages have a significantly reduced capacity to bind syngeneic lymphoid cells. The difference in adhesivity between intact and stimulated PEC can be abolished by glutaraldehyde prefixation. In vivo induction results in modified morphological and functional properties of macrophages, including transformation of their cell surface.
约5%至6%的小鼠腹腔渗出细胞（PEC）能自发地与绵羊红细胞（SRBC）形成玫瑰花结。这种微弱的细胞间相互作用很可能是由位于腹腔巨噬细胞表面的SRBC“受体”介导的。该受体的表现受PEC供体的遗传背景和H-2单倍型影响；高比例的红细胞玫瑰花结形成细胞（RFC）与H-2s单倍型相关。来自完整小鼠的PEC在形态和功能特征上与经腹腔注射百日咳杆菌（PP）或破伤风类毒素（TG）预处理的供体的PEC明显不同。诱导巨噬细胞形成玫瑰花结取决于刺激与收获PEC之间的时间间隔以及诱导剂的类型；它对辐射也敏感，且对秋水仙碱的作用更敏感。TG诱导的巨噬细胞结合同基因淋巴细胞的能力显著降低。完整PEC与刺激后PEC之间的黏附性差异可通过戊二醛预固定消除。体内诱导会导致巨噬细胞形态和功能特性的改变，包括其细胞表面的转化。
