Beller D I, Kiely J M, Unanue E R
J Immunol. 1980 Mar;124(3):1426-32.
The amounts of Ia-positive and -negative macrophages were studied in peritoneal exudates of normal mice or of mice injected with various inflammatory materials, infected with Listeria monocytogenes, or injected with hemocyanin. Ia-negative macrophages predominated in exudates from normal mice or from mice given mineral oil, peptone, thioglycollate, culture media, or endotoxin. Infection with Listeria caused a very marked increase in Ia-positive macrophages. The induction of Ia-positive macrophages by Listeria inoculation resulted in great part from an immune process. The Ia-positive exudates were more readily generated in immune mice given a secondary challenge with heat-killed organisms. Furthermore, immune T cells transplanted together with heat-killed organisms into normal mice resulted in Ia-rich exudates. Injection of hemocyanin also induced Ia-rich exudates involving an immune process. We conclude that an immune reaction involving T cells regulates the Ia phenotype of the exudate macrophage population. The Ia-positive macrophages were Fc and C3 receptor positive and phagocytized latex particles.
研究了正常小鼠或注射各种炎性物质、感染单核细胞增生李斯特菌或注射血蓝蛋白的小鼠腹腔渗出液中Ia阳性和阴性巨噬细胞的数量。Ia阴性巨噬细胞在正常小鼠或给予矿物油、蛋白胨、巯基乙酸盐、培养基或内毒素的小鼠的渗出液中占主导。感染李斯特菌导致Ia阳性巨噬细胞显著增加。接种李斯特菌诱导Ia阳性巨噬细胞很大程度上源于免疫过程。在用热灭活的生物体进行二次攻击的免疫小鼠中，更容易产生Ia阳性渗出液。此外，将免疫T细胞与热灭活的生物体一起移植到正常小鼠中会导致富含Ia的渗出液。注射血蓝蛋白也会诱导涉及免疫过程的富含Ia的渗出液。我们得出结论，涉及T细胞的免疫反应调节渗出液巨噬细胞群体的Ia表型。Ia阳性巨噬细胞为Fc和C3受体阳性，并吞噬乳胶颗粒。
