Liver regeneration: An isolation perfusion system employed to assay hepatic 3-H-thymidine incorporation.

Isolated normal livers perfused with 3-H-thymidine containing suspensions which had been previously circulated through isolated livers either (a) regenerating or (b) "sham" operated, showed equal and erlatively low levels of both tissue specific activity and nuclear labeling by autoradiography. When such blood-simulating perfusates, containing 3-H-thymidine, are circulated through whole isolated regenerating livers, nuclear uptake is apparent and specific activity is increased greater than 100 times over levels obtained when the same perfusate is circulated through nonregenerating livers.