Vander Borght T, Lambotte L, Pauwels S, Labar D, Beckers C, Dive C
Department of Gastroenterology, University of Louvain Medical School, Brussels, Belgium.
Gastroenterology. 1991 Sep;101(3):794-9. doi: 10.1016/0016-5085(91)90541-r.
The feasibility of liver regeneration determination with [2-11C]thymidine and positron emission tomography was investigated in partially hepatectomized rats. Serial tomographic scans were performed over a 120-minute period after injection of [2-11C]thymidine together with tritium-labeled thymidine. Within 10 minutes after injection, positron emission tomography scans showed a twofold higher hepatic uptake in regenerating than in nonregenerating livers. Time-activity curves over the liver area indicated that the maximal uptake was followed by a faster decrease of 11C radioactivity in controls than in regenerating animals, so that total 11C activity remaining in the liver at 120 minutes accounted for 68% of maximum in regenerating and only 38% in controls. Tissue distribution studies performed at 120 minutes showed that total 11C radioactivity, expressed in percent injected dose per gram, was six times higher in regenerating livers than in controls (0.62% +/- 0.07% in regenerating livers and 0.10% +/- 0.03% in nonregenerating livers; P less than 0.001) and correlated with 3H radioactivity measured in the nuclear fraction (r = 0.92; P less than 0.001). When the hepatic uptake was expressed in percent of dose per organ, the difference between both groups increased (2.31% +/- 0.23% in regenerating livers and 0.29% +/- 0.02% in nonregenerating livers; P less than 0.001) because of higher weight of regenerating livers than of nonregenerating livers (3.83 +/- 0.11 g in regenerating livers and 2.96 +/- 0.16 g in nonregenerating livers; P less than 0.001). In other organs examined, no difference in 11C radioactivity was found between the two groups of rats. These results indicated the potential usefulness of [2-11C]thymidine and positron emission tomography for noninvasive measurement of liver regeneration.
在部分肝切除的大鼠中，研究了用[2-11C]胸腺嘧啶核苷和正电子发射断层扫描测定肝再生的可行性。注射[2-11C]胸腺嘧啶核苷和氚标记的胸腺嘧啶核苷后，在120分钟内进行了系列断层扫描。注射后10分钟内，正电子发射断层扫描显示，再生肝脏中的肝摄取量比未再生肝脏高两倍。肝脏区域的时间-活性曲线表明，对照组中11C放射性的最大摄取量之后下降速度比再生动物更快，因此在120分钟时肝脏中剩余的总11C活性在再生组中占最大值的68%，而在对照组中仅占38%。在120分钟时进行的组织分布研究表明，以每克注射剂量的百分比表示的总11C放射性，再生肝脏比对照组高六倍（再生肝脏中为0.62%±0.07%，未再生肝脏中为0.10%±0.03%；P<0.001），并且与在核部分中测量的3H放射性相关（r = 0.92；P<0.001）。当以每器官剂量的百分比表示肝摄取量时，两组之间的差异增大（再生肝脏中为2.31%±0.23%，未再生肝脏中为0.29%±0.02%；P<0.001），因为再生肝脏的重量比未再生肝脏的重量更大（再生肝脏中为3.83±0.11克，未再生肝脏中为2.96±0.16克；P<0.001）。在检查的其他器官中，两组大鼠之间未发现11C放射性有差异。这些结果表明[2-11C]胸腺嘧啶核苷和正电子发射断层扫描在无创测量肝再生方面具有潜在的实用性。
